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. 1990 Jan;92(1):151–154. doi: 10.1104/pp.92.1.151

Energy Requirement for the Import of Protein into Plastids from Developing Endosperm of Ricinus communis L. 1

Sheila A Boyle 1, Sean M Hemmingsen 1, David T Dennis 1
PMCID: PMC1062262  PMID: 16667238

Abstract

Leucoplasts isolated from developing endosperm of Ricinus communis L. will import the precursor of the small subunit of ribulose bisphosphate carboxylase from pea shoots and process it to its mature molecular weight (SA Boyle, SM Hemmingsen, DT Dennis [1986] Plant Physiol 81: 817-822). This process requires energy in the form of ATP. GTP, CTP, and UTP are inactive. ADP will also satisfy the energy requirement, probably through the action of adenylate kinase in the envelope. Fatty acid biosynthesis which occurs within these leucoplasts also requires ATP for maximal activity. Phosphoenolpyruvate will stimulate fatty acid biosynthesis approximately three times as effectively as ATP through the generation of ATP within the organelle by the action of the plastid pyruvate kinase. However, phosphoenolpyruvate under similar conditions will not stimulate the uptake of the small subunit of ribulose bisphosphate carboxylase into leucoplasts. These results indicate that ATP is required outside the leucoplast for protein uptake and that internally generated ATP is not effective in this process.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benedict C. R. The presence of ribulose 1,5-diphosphate carboxylase in the nonphotosynthetic endosperm of germinating castor beans. Plant Physiol. 1973 Apr;51(4):755–759. doi: 10.1104/pp.51.4.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyle S. A., Hemmingsen S. M., Dennis D. T. Uptake and processing of the precursor to the small subunit of ribulose 1,5-bisphosphate carboxylase by leucoplasts from the endosperm of developing castor oil seeds. Plant Physiol. 1986 Jul;81(3):817–822. doi: 10.1104/pp.81.3.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cashmore A. R., Broadhurst M. K., Gray R. E. Cell-free synthesis of leaf protein: Identification of an apparent precursor of the small subunit of ribulose-1,5-bisphosphate carboxylase. Proc Natl Acad Sci U S A. 1978 Feb;75(2):655–659. doi: 10.1073/pnas.75.2.655. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cline K., Werner-Washburne M., Lubben T. H., Keegstra K. Precursors to two nuclear-encoded chloroplast proteins bind to the outer envelope membrane before being imported into chloroplasts. J Biol Chem. 1985 Mar 25;260(6):3691–3696. [PubMed] [Google Scholar]
  5. Eilers M., Oppliger W., Schatz G. Both ATP and an energized inner membrane are required to import a purified precursor protein into mitochondria. EMBO J. 1987 Apr;6(4):1073–1077. doi: 10.1002/j.1460-2075.1987.tb04860.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eilers M., Schatz G. Protein unfolding and the energetics of protein translocation across biological membranes. Cell. 1988 Feb 26;52(4):481–483. doi: 10.1016/0092-8674(88)90458-8. [DOI] [PubMed] [Google Scholar]
  7. Finlayson S. A., Dennis D. T. Acetyl-coenzyme A carboxylase from the developing endosperm of Ricinus communis. II. A two-site kinetic mechanism. Arch Biochem Biophys. 1983 Sep;225(2):586–595. doi: 10.1016/0003-9861(83)90070-x. [DOI] [PubMed] [Google Scholar]
  8. Flügge U. I., Hinz G. Energy dependence of protein translocation into chloroplasts. Eur J Biochem. 1986 Nov 3;160(3):563–570. doi: 10.1111/j.1432-1033.1986.tb10075.x. [DOI] [PubMed] [Google Scholar]
  9. Ireland R. J., Deluca V., Dennis D. T. Isoenzymes of pyruvate kinase in etioplasts and chloroplasts. Plant Physiol. 1979 May;63(5):903–907. doi: 10.1104/pp.63.5.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Keegstra K., Bauerle C. Targeting of proteins into chloroplasts. Bioessays. 1988 Jul;9(1):15–19. doi: 10.1002/bies.950090105. [DOI] [PubMed] [Google Scholar]
  11. Keegstra K. Transport and routing of proteins into chloroplasts. Cell. 1989 Jan 27;56(2):247–253. doi: 10.1016/0092-8674(89)90898-2. [DOI] [PubMed] [Google Scholar]
  12. Miernyk J. A., Dennis D. T. Isozymes of the glycolytic enzymes in endosperm from developing castor oil seeds. Plant Physiol. 1982 Apr;69(4):825–828. doi: 10.1104/pp.69.4.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Murakami S., Strotmann H. Adenylate kinase bound to the envelope membranes of spinach chloroplasts. Arch Biochem Biophys. 1978 Jan 15;185(1):30–38. doi: 10.1016/0003-9861(78)90140-6. [DOI] [PubMed] [Google Scholar]
  14. Olsen L. J., Theg S. M., Selman B. R., Keegstra K. ATP is required for the binding of precursor proteins to chloroplasts. J Biol Chem. 1989 Apr 25;264(12):6724–6729. [PubMed] [Google Scholar]
  15. Pain D., Blobel G. Protein import into chloroplasts requires a chloroplast ATPase. Proc Natl Acad Sci U S A. 1987 May;84(10):3288–3292. doi: 10.1073/pnas.84.10.3288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pfisterer J., Lachmann P., Kloppstech K. Transport of proteins into chloroplasts. Binding of nuclear-coded chloroplast proteins to the chloroplast envelope. Eur J Biochem. 1982 Aug;126(1):143–148. doi: 10.1111/j.1432-1033.1982.tb06758.x. [DOI] [PubMed] [Google Scholar]
  17. Schindler C., Soll J. Protein transport in intact, purified pea etioplasts. Arch Biochem Biophys. 1986 May 15;247(1):211–220. doi: 10.1016/0003-9861(86)90550-3. [DOI] [PubMed] [Google Scholar]
  18. Simcox P. D., Reid E. E., Canvin D. T., Dennis D. T. Enzymes of the Glycolytic and Pentose Phosphate Pathways in Proplastids from the Developing Endosperm of Ricinus communis L. Plant Physiol. 1977 Jun;59(6):1128–1132. doi: 10.1104/pp.59.6.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Theg S. M., Bauerle C., Olsen L. J., Selman B. R., Keegstra K. Internal ATP is the only energy requirement for the translocation of precursor proteins across chloroplastic membranes. J Biol Chem. 1989 Apr 25;264(12):6730–6736. [PubMed] [Google Scholar]

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