Skip to main content
NCBI home page
Plant Physiology logoLink to Plant Physiology
. 1990 Jan;92(1):276–280. doi: 10.1104/pp.92.1.276

Differential Activation of Expression of a Suberization-Associated Anionic Peroxidase Gene in Near-Isogenic Resistant and Susceptible Tomato Lines by Elicitors of Verticillium albo-atratrum1

Royce Mohan 1, Pappachan E Kolattukudy 1
PMCID: PMC1062283  PMID: 16667260

Abstract

We tested whether the expression of the suberization-associated anionic peroxidase gene is involved in the timely appearance of the vascular suberized coating involved in the resistance of a tomato line to Verticillium albo-atrum. The mRNA for this peroxidase appeared at a higher level one day earlier in wound-healing fruits of the resistant tomato line than in a near-isogenic susceptible line. Cell cultures from the resistant line, when treated with low levels (nanograms per milliliter) of fungal elicitor, generated the peroxidase mRNA and this apparent activation of the peroxidase gene expression could be detected in minutes, whereas the cells from the susceptible line hardly responded.

Full text

PDF
276

Images in this article

277Figure 1
on p.277
278Figure 2
on p.278
278Figure 3
on p.278

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson-Prouty A. J., Albersheim P. Host-Pathogen Interactions: VIII. Isolation of a Pathogen-synthesized Fraction Rich in Glucan That Elicits a Defense Response in the Pathogen's Host. Plant Physiol. 1975 Aug;56(2):286–291. doi: 10.1104/pp.56.2.286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Borchert R. Time course and spatial distribution of phenylalanine ammonia-lyase and peroxidase activity in wounded potato tuber tissue. Plant Physiol. 1978 Nov;62(5):789–793. doi: 10.1104/pp.62.5.789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Espelie K. E., Franceschi V. R., Kolattukudy P. E. Immunocytochemical localization and time course of appearance of an anionic peroxidase associated with suberization in wound-healing potato tuber tissue. Plant Physiol. 1986 Jun;81(2):487–492. doi: 10.1104/pp.81.2.487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Espelie K. E., Kolattukudy P. E. Purification and characterization of an abscisic acid-inducible anionic peroxidase associated with suberization in potato (Solanum tuberosum). Arch Biochem Biophys. 1985 Aug 1;240(2):539–545. doi: 10.1016/0003-9861(85)90060-8. [DOI] [PubMed] [Google Scholar]
  6. Everdeen D. S., Kiefer S., Willard J. J., Muldoon E. P., Dey P. M., Li X. B., Lamport D. T. Enzymic cross-linkage of monomeric extensin precursors in vitro. Plant Physiol. 1988 Jul;87(3):616–621. doi: 10.1104/pp.87.3.616. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hankin L., Kolattukudy P. E. Metabolism of a plant wax paraffin (n-nonacosane) by a soil bacterium (Micrococcus cerificans). J Gen Microbiol. 1968 May;51(3):457–463. doi: 10.1099/00221287-51-3-457. [DOI] [PubMed] [Google Scholar]
  8. Kolattukudy P. E. Biopolyester membranes of plants: cutin and suberin. Science. 1980 May 30;208(4447):990–1000. doi: 10.1126/science.208.4447.990. [DOI] [PubMed] [Google Scholar]
  9. Lagrimini L. M., Rothstein S. Tissue specificity of tobacco peroxidase isozymes and their induction by wounding and tobacco mosaic virus infection. Plant Physiol. 1987 Jun;84(2):438–442. doi: 10.1104/pp.84.2.438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Roberts E., Kolattukudy P. E. Molecular cloning, nucleotide sequence, and abscisic acid induction of a suberization-associated highly anionic peroxidase. Mol Gen Genet. 1989 Jun;217(2-3):223–232. doi: 10.1007/BF02464885. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES