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. 1990 Feb;92(2):462–466. doi: 10.1104/pp.92.2.462

Effect of Polyethylene Glycol Exclusion on the Water Potential of Solution-Saturated Filter Paper

Stuart P Hardegree 1,2, William E Emmerich 1,2
PMCID: PMC1062314  PMID: 16667298

Abstract

Solutions of high molecular weight polyethylene glycol are often used to control water potential in seed germination studies. There is an implicit assumption that the seed support materials do not alter the water potential of the osmotic solution. Filter paper, however, contains a hydrophylic volume fraction that is inaccessible to high molecular weight polymers. Water absorbed by filter paper fibers was found to concentrate polyethylene glycol and lower water potential in solution-filter paper mixtures. The magnitude of this concentration effect is a function of the original concentration of polyethylene glycol and the ratio of solution volume to filter paper weight.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carpita N., Sabularse D., Montezinos D., Delmer D. P. Determination of the pore size of cell walls of living plant cells. Science. 1979 Sep 14;205(4411):1144–1147. doi: 10.1126/science.205.4411.1144. [DOI] [PubMed] [Google Scholar]
  2. Michel B. E. Evaluation of the water potentials of solutions of polyethylene glycol 8000 both in the absence and presence of other solutes. Plant Physiol. 1983 May;72(1):66–70. doi: 10.1104/pp.72.1.66. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Michel B. E. Further Comparisons between Carbowax 6000 and Mannitol as Suppressants of Cucumber Hypocotyl Elongation. Plant Physiol. 1971 Nov;48(5):513–516. doi: 10.1104/pp.48.5.513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Michel B. E., Kaufmann M. R. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 1973 May;51(5):914–916. doi: 10.1104/pp.51.5.914. [DOI] [PMC free article] [PubMed] [Google Scholar]

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