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. 1990 Mar;92(3):684–689. doi: 10.1104/pp.92.3.684

On The Regulation of Spinach Nitrate Reductase 1

Juan Sanchez 1,2, Hans W Heldt 1
PMCID: PMC1062354  PMID: 16667335

Abstract

A coupled assay has been worked out to study spinach (Spinacea oleracea L.) nitrate reductase under low, more physiological concentrations of NADH. In this assay the reduction of nitrate is coupled to the oxidation of malate catalyzed by spinach NAD-malate dehydrogenase. The use of this coupled system allows the assay of nitrate reductase activity at steady-state concentrations of NADH below micromolar. We have used this coupled assay to study the kinetic parameters of spinach nitrate reductase and to reinvestigate the putative regulatory role of adenine nucleotides, inorganic phosphate, amino acids, and calcium and calmodulin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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