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. 1990 Apr;92(4):1081–1085. doi: 10.1104/pp.92.4.1081

A Mutation Causing Imidazolinone Resistance Maps to the Csr1 Locus of Arabidopsis thaliana1

George W Haughn 1,2, Christopher R Somerville 1,2
PMCID: PMC1062419  PMID: 16667374

Abstract

A mutant of Arabidopsis thaliana, two hundred times more resistant to the imidazolinone herbicide imazapyr than wild-type plants, was isolated by direct selection of seedlings from a mutagenized population. Genetic analysis showed that resistance is due to a single dominant nuclear mutation that could not be separated by recombination from a mutation in the CSR1 gene encoding acetohydroxy acid synthase. Acetohydroxy acid synthase activity in extracts isolated from the mutant was 1000-fold more resistant to inhibition by imazapyr than that of the wild type. The resistant enzyme activity cosegregated with whole plant resistance. These data strongly suggest that the mutation is an allele of CSR1 encoding an imazapyr-resistant AHAS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  2. Chaleff R. S., Mauvais C. J. Acetolactate synthase is the site of action of two sulfonylurea herbicides in higher plants. Science. 1984 Jun 29;224(4656):1443–1445. doi: 10.1126/science.224.4656.1443. [DOI] [PubMed] [Google Scholar]
  3. Chaleff R. S., Ray T. B. Herbicide-resistant mutants from tobacco cell cultures. Science. 1984 Mar 16;223(4641):1148–1151. doi: 10.1126/science.223.4641.1148. [DOI] [PubMed] [Google Scholar]
  4. Chang C., Bowman J. L., DeJohn A. W., Lander E. S., Meyerowitz E. M. Restriction fragment length polymorphism linkage map for Arabidopsis thaliana. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6856–6860. doi: 10.1073/pnas.85.18.6856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Erickson J. M., Rahire M., Rochaix J. D., Mets L. Herbicide resistance and cross-resistance: changes at three distinct sites in the herbicide-binding protein. Science. 1985 Apr 12;228(4696):204–207. doi: 10.1126/science.228.4696.204. [DOI] [PubMed] [Google Scholar]
  6. Falco S. C., Dumas K. S. Genetic analysis of mutants of Saccharomyces cerevisiae resistant to the herbicide sulfometuron methyl. Genetics. 1985 Jan;109(1):21–35. doi: 10.1093/genetics/109.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hagberg L., Hull R., Hull S., Falkow S., Freter R., Svanborg Edén C. Contribution of adhesion to bacterial persistence in the mouse urinary tract. Infect Immun. 1983 Apr;40(1):265–272. doi: 10.1128/iai.40.1.265-272.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lee K. Y., Townsend J., Tepperman J., Black M., Chui C. F., Mazur B., Dunsmuir P., Bedbrook J. The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO J. 1988 May;7(5):1241–1248. doi: 10.1002/j.1460-2075.1988.tb02937.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mazur B. J., Chui C. F., Smith J. K. Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides. Plant Physiol. 1987 Dec;85(4):1110–1117. doi: 10.1104/pp.85.4.1110. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saxena P. K., King J. Herbicide Resistance in Datura innoxia: Cross-Resistance of Sulfonylurea-Resistant Cell Lines to Imidazolinones. Plant Physiol. 1988 Mar;86(3):863–867. doi: 10.1104/pp.86.3.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Shaner D. L., Anderson P. C., Stidham M. A. Imidazolinones: potent inhibitors of acetohydroxyacid synthase. Plant Physiol. 1984 Oct;76(2):545–546. doi: 10.1104/pp.76.2.545. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Suiter K. A., Wendel J. F., Case J. S. LINKAGE-1: a PASCAL computer program for the detection and analysis of genetic linkage. J Hered. 1983 May-Jun;74(3):203–204. doi: 10.1093/oxfordjournals.jhered.a109766. [DOI] [PubMed] [Google Scholar]
  13. Winder T., Spalding M. H. Imazaquin and chlorsulfuron resistance and cross resistance in mutants of Chlamydomonas reinhardtii. Mol Gen Genet. 1988 Aug;213(2-3):394–399. doi: 10.1007/BF00339608. [DOI] [PubMed] [Google Scholar]
  14. Yadav N., McDevitt R. E., Benard S., Falco S. C. Single amino acid substitutions in the enzyme acetolactate synthase confer resistance to the herbicide sulfometuron methyl. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4418–4422. doi: 10.1073/pnas.83.12.4418. [DOI] [PMC free article] [PubMed] [Google Scholar]

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