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. 1990 May;93(1):216–221. doi: 10.1104/pp.93.1.216

Diamine Oxidase from Cultured Roots of Hyoscyamus niger

Its Function in Tropane Alkaloid Biosynthesis

Takashi Hashimoto 1, Akira Mitani 1, Yasuyuki Yamada 1
PMCID: PMC1062491  PMID: 16667438

Abstract

Diamine oxidase was partially purified from cultured roots of Hyoscyamus niger L. that produce considerable amounts of tropane alkaloids, and then characterized. N-Methylated amines inhibited the activity of the enzyme more strongly than the corresponding primary amines. N-Methylputrescine was the best substrate of those studied, the respective Km values for it and for putrescine and cadaverine being 0.33, 2.85, and 6.25 millimolar. The specificity constants Vmax/Km for putrescine and cadaverine were 11 and 1% of the constant for N-methylputrescine. Marked specificity for the N-methylated diamine would enable the Hyoscyamus enzyme to function specifically in tropane alkaloid biosynthesis.

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Selected References

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