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. 1990 Jun;93(2):739–747. doi: 10.1104/pp.93.2.739

Immunodetection of Protein Glycoforms Encoded by Two Independent Genes of the Self-Incompatibility Multigene Family of Brassica1

Ann L Umbach 1, Beth A Lalonde 1, Muthugapatti K Kandasamy 1, June B Nasrallah 1, Mikhail E Nasrallah 1
PMCID: PMC1062578  PMID: 16667531

Abstract

Glycoprotein products of two highly homologous Brassica S gene family members were studied: SLSG (S locus-specific glycoprotein), product of an SLG gene at the S locus, and SLR1 (S locus-related) protein, product of the SLR1 gene, a gene unlinked to the S locus. A polyclonal antibody directed against a trpE-SLR1 fusion protein facilitated study of the SLR1 protein. SLR1 protein was detected in a number of crucifer species. No variation in the level of this protein was found between self-compatible and self-incompatible plants. Both SLSG and SLR1 protein occurred as glycoforms on sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. Each glycoform had several charge forms, indicated by elution patterns from a high performance liquid chromatography cation exchange column and behavior on two-dimensional gels. Deglycosylation of both SLSG and SLR1 protein caused loss of the glycoforms, which apparently arose from differences in glycosylation. Consistent with their apparent similar post-translational processing, immunolocalization showed that SLR1 protein, like SLSG, accumulated in the stigma papillae cell walls. Thus, both SLSG and SLR1 protein are present at the site of pollen-stigma interaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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