Abstract
A biotype of Stellaria media (L.) Vill. has been identified that is highly resistant to the herbicide chlorsulfuron. Resistance is due to an altered acetolactate synthase (ALS) that is much less sensitive to chlorsulfuron than the ALS from the susceptible (S) biotype. The S biotype was extremely sensitive to D489 (N-[2,6-dichlorophenyl]-5,7-dimethyl-1,2,4-triazolo[1,5a] pyrimidine-2-sulfonamide), a member of a new class of triazolopyrimidine herbicides, while the chlorsulfuron-resistant biotype exhibited complete cross-resistance at both the whole plant and enzyme levels. ALS activity of the S biotype was reduced by approximately 90% in the presence of 0.1 micromolar D489, while that of the R biotype was reduced by less than 10%. This result suggests that the two herbicides share a common binding site on ALS. Only very slight cross-resistance at the ALS level was found to imazamethabenz, an imidazolinone herbicide.
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Selected References
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- Chaleff R. S., Ray T. B. Herbicide-resistant mutants from tobacco cell cultures. Science. 1984 Mar 16;223(4641):1148–1151. doi: 10.1126/science.223.4641.1148. [DOI] [PubMed] [Google Scholar]
- Falco S. C., Dumas K. S. Genetic analysis of mutants of Saccharomyces cerevisiae resistant to the herbicide sulfometuron methyl. Genetics. 1985 Jan;109(1):21–35. doi: 10.1093/genetics/109.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartnett M. E., Newcomb J. R., Hodson R. C. Mutations in Chlamydomonas reinhardtii Conferring Resistance to the Herbicide Sulfometuron Methyl. Plant Physiol. 1987 Dec;85(4):898–901. doi: 10.1104/pp.85.4.898. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LaRossa R. A., Schloss J. V. The sulfonylurea herbicide sulfometuron methyl is an extremely potent and selective inhibitor of acetolactate synthase in Salmonella typhimurium. J Biol Chem. 1984 Jul 25;259(14):8753–8757. [PubMed] [Google Scholar]
- Mazur B. J., Chui C. F., Smith J. K. Isolation and characterization of plant genes coding for acetolactate synthase, the target enzyme for two classes of herbicides. Plant Physiol. 1987 Dec;85(4):1110–1117. doi: 10.1104/pp.85.4.1110. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Muhitch M. J., Shaner D. L., Stidham M. A. Imidazolinones and acetohydroxyacid synthase from higher plants: properties of the enzyme from maize suspension culture cells and evidence for the binding of imazapyr to acetohydroxyacid synthase in vivo. Plant Physiol. 1987 Feb;83(2):451–456. doi: 10.1104/pp.83.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ray T. B. Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol. 1984 Jul;75(3):827–831. doi: 10.1104/pp.75.3.827. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saxena P. K., King J. Herbicide Resistance in Datura innoxia: Cross-Resistance of Sulfonylurea-Resistant Cell Lines to Imidazolinones. Plant Physiol. 1988 Mar;86(3):863–867. doi: 10.1104/pp.86.3.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winder T., Spalding M. H. Imazaquin and chlorsulfuron resistance and cross resistance in mutants of Chlamydomonas reinhardtii. Mol Gen Genet. 1988 Aug;213(2-3):394–399. doi: 10.1007/BF00339608. [DOI] [PubMed] [Google Scholar]
- Yadav N., McDevitt R. E., Benard S., Falco S. C. Single amino acid substitutions in the enzyme acetolactate synthase confer resistance to the herbicide sulfometuron methyl. Proc Natl Acad Sci U S A. 1986 Jun;83(12):4418–4422. doi: 10.1073/pnas.83.12.4418. [DOI] [PMC free article] [PubMed] [Google Scholar]