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. 1990 Aug;93(4):1312–1315. doi: 10.1104/pp.93.4.1312

Properties of a Mutant from Synechocystis PCC6803 Resistant to Acetazolamide, an Inhibitor of Carbonic Anhydrase 1

Sylvie Bédu 1,2, Gilles Peltier 1,2, Frédéric Sarrey 1,2, Françoise Joset 1,2
PMCID: PMC1062673  PMID: 16667618

Abstract

A spontaneous mutant of the cyanobacterium Synechocystis PCC6803 was isolated for its resistance to acetazolamide, an inhibitor of carbonic anhydrase. The mutant showed a deficiency in oxygen exchange between CO2 and H2O, a lower level of stable internal CO2 pool and a decreased capacity to adapt its photosynthetic affinity under limited inorganic carbon regime. The initial rate of uptake of inorganic carbon was identical to that of wild-type cells. It is demonstrated that the mutation affects the carbonic anhydrase activity. This could result from either of two impairments: a deficiency in the enzyme activity detectable by mass spectrometric determinations, or a modification of the cellular compartment in which the enzyme is located, preventing its activity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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