Abstract
Exposure of plant tissue culture media to light from fluorescent bulbs changed the growth regulating properties of the media. The light caused nutrient medium-dependent photosensitized degradation of the phytohormone indole-3-acetic acid and other media components. Photochemical changes in culture media were caused by light from 290 to 450 nanometers and were prevented with a yellow long-pass filter. The use of appropriately filtered light when culturing plant material can eliminate unnecessary variability by stabilizing the culture media composition.
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Selected References
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- Dunlap J. R., Kresovich S., McGee R. E. The effect of salt concentration on auxin stability in culture media. Plant Physiol. 1986 Jul;81(3):934–936. doi: 10.1104/pp.81.3.934. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dunlap J. R., Robacker K. M. Nutrient salts promote light-induced degradation of indole-3-acetic Acid in tissue culture media. Plant Physiol. 1988 Oct;88(2):379–382. doi: 10.1104/pp.88.2.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hangarter R. P., Good N. E. Evidence That IAA Conjugates Are Slow-Release Sources of Free IAA in Plant Tissues. Plant Physiol. 1981 Dec;68(6):1424–1427. doi: 10.1104/pp.68.6.1424. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hangarter R. P., Peterson M. D., Good N. E. Biological activities of indoleacetylamino acids and their use as auxins in tissue culture. Plant Physiol. 1980 May;65(5):761–767. doi: 10.1104/pp.65.5.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klein R. M., Edsall P. C. Interference by near ultraviolet and green light with growth of animal and plant cell cultures. Photochem Photobiol. 1967 Nov;6(11):841–850. doi: 10.1111/j.1751-1097.1967.tb08897.x. [DOI] [PubMed] [Google Scholar]
- Park R. D., Park C. K. Oxidation of indole-3-acetic Acid-amino Acid conjugates by horseradish peroxidase. Plant Physiol. 1987 Jul;84(3):826–829. doi: 10.1104/pp.84.3.826. [DOI] [PMC free article] [PubMed] [Google Scholar]