Abstract
Free indole-3-acetic acid levels were measured by gas chromatography-mass spectrometry in three ultra-tall `slender' Pisum sativum L. lines differing in gibberellin content. Measurements were made for apices and stem elongation zones of light-grown plants and values were compared with wild-type, dwarf, and nana phenotypes in which internode length is genetically regulated, purportedly via the gibberellin level. Indole-3-acetic acid levels of growing stems paralleled growth rates in all lines, and were high in all three slender genotypes. Growth was inhibited by p-chlorophenoxyisobutyric acid, demonstrating the requirement of auxin activity for stem elongation, and also by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid. It is concluded that the slender phenotype may arise from constant activation of a gibberellin receptor or transduction chain event leading directly or indirectly to elevated levels of indole-3-acetic acid, and that increased indole-3-acetic acid levels are a significant factor in the promotion of stem elongation.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bandurski R. S., Schulze A. Concentration of Indole-3-acetic Acid and Its Derivatives in Plants. Plant Physiol. 1977 Aug;60(2):211–213. doi: 10.1104/pp.60.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cohen J. D., Baldi B. G., Slovin J. P. C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants. Plant Physiol. 1986 Jan;80(1):14–19. doi: 10.1104/pp.80.1.14. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ingram T. J., Reid J. B. Internode Length in Pisum: Gene na May Block Gibberellin Synthesis between ent-7alpha-Hydroxykaurenoic Acid and Gibberellin A(12)-Aldehyde. Plant Physiol. 1987 Apr;83(4):1048–1053. doi: 10.1104/pp.83.4.1048. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Law D. M., Hamilton R. H. A rapid isotope dilution method for analysis of indole-3-acetic acid and indoleacetyl aspartic acid from small amounts of plant tissue. Biochem Biophys Res Commun. 1982 Jun 15;106(3):1035–1041. doi: 10.1016/0006-291x(82)91815-0. [DOI] [PubMed] [Google Scholar]
- Law D. M., Hamilton R. H. Effects of gibberellic Acid on endogenous indole-3-acetic Acid and indoleacetyl aspartic Acid levels in a dwarf pea. Plant Physiol. 1984 May;75(1):255–256. doi: 10.1104/pp.75.1.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas R. J., Harrison M. A., Taylor J., Kaufman P. B. Endogenous auxin and ethylene in pellia (bryophyta). Plant Physiol. 1983 Oct;73(2):395–397. doi: 10.1104/pp.73.2.395. [DOI] [PMC free article] [PubMed] [Google Scholar]