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. 1990 Aug;93(4):1610–1619. doi: 10.1104/pp.93.4.1610

Wall Extensibility and Cell Hydraulic Conductivity Decrease in Enlarging Stem Tissues at Low Water Potentials 1

Hiroshi Nonami 1,2,2, John S Boyer 1,2
PMCID: PMC1062719  PMID: 16667664

Abstract

Measurements with a guillotine psychrometer (H Nonami, JS Boyer [1990] Plant Physiol 94: 1601-1609) indicate that the inhibition of stem growth at low water potentials (low ψw) is accompanied by decreases in cell wall extensibility and tissue hydraulic conductance to water that eventually limit growth rate in soybean (Glycine max L. Merr.). To check this conclusion, we measured cell wall properties and cell hydraulic conductivities with independent techniques in soybean seedlings grown and treated the same way, i.e. grown in the dark and exposed to low ψw by transplanting dark grown seedlings to vermiculite of low water content. Wall properties were measured with an extensiometer modified for intact plants, and conductances were measured with a cell pressure probe in intact plants. Theory was developed to relate the wall measurements to those with the psychrometer. In the elongation zone, the plastic deformability of the walls decreased when measured with the extensiometer while growth was inhibited at low ψw. It increased during a modest growth recovery. This behavior was the same as that for the wall extensibility observed previously with the psychrometer. Tissue that was killed before measurement with the extensiometer also showed a similar response, indicating that changes in wall extensibility represented changes in wall physical properties and not rates of wall biosynthesis. The elastic compliance (reciprocal of bulk elastic modulus) did not change in the elongating or mature tissue. The hydraulic conductivity of cortical cells decreased in the elongating tissue and increased slightly during growth recovery in a response similar to that observed with the psychrometer. We conclude that the plastic properties of the cell walls and the conductance of the cells to water were decreased at low ψw but that the elastic properties of the walls were of little consequence in this response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bensen R. J., Boyer J. S., Mullet J. E. Water deficit-induced changes in abscisic Acid, growth, polysomes, and translatable RNA in soybean hypocotyls. Plant Physiol. 1988 Oct;88(2):289–294. doi: 10.1104/pp.88.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boyer J. S. Leaf enlargement and metabolic rates in corn, soybean, and sunflower at various leaf water potentials. Plant Physiol. 1970 Aug;46(2):233–235. doi: 10.1104/pp.46.2.233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bozarth C. S., Mullet J. E., Boyer J. S. Cell wall proteins at low water potentials. Plant Physiol. 1987 Sep;85(1):261–267. doi: 10.1104/pp.85.1.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bressan R. A., Handa A. K., Handa S., Hasegawa P. M. Growth and water relations of cultured tomato cells after adjustment to low external water potentials. Plant Physiol. 1982 Nov;70(5):1303–1309. doi: 10.1104/pp.70.5.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cosgrove D. J., Cleland R. E. Osmotic properties of pea internodes in relation to growth and auxin action. Plant Physiol. 1983 Jun;72(2):332–338. doi: 10.1104/pp.72.2.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Green P. B., Cummins W. R. Growth rate and turgor pressure: auxin effect studies with an automated apparatus for single coleoptiles. Plant Physiol. 1974 Dec;54(6):863–869. doi: 10.1104/pp.54.6.863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hüsken D., Steudle E., Zimmermann U. Pressure probe technique for measuring water relations of cells in higher plants. Plant Physiol. 1978 Feb;61(2):158–163. doi: 10.1104/pp.61.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mason H. S., Mullet J. E., Boyer J. S. Polysomes, Messenger RNA, and Growth in Soybean Stems during Development and Water Deficit. Plant Physiol. 1988 Mar;86(3):725–733. doi: 10.1104/pp.86.3.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Matyssek R., Maruyama S., Boyer J. S. Rapid wall relaxation in elongating tissues. Plant Physiol. 1988 Apr;86(4):1163–1167. doi: 10.1104/pp.86.4.1163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Molz F. J. Growth-induced Water Potentials in Plant Cells and Tissues. Plant Physiol. 1978 Sep;62(3):423–429. doi: 10.1104/pp.62.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Nonami H., Boyer J. S. Primary events regulating stem growth at low water potentials. Plant Physiol. 1990 Aug;93(4):1601–1609. doi: 10.1104/pp.93.4.1601. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nonami H., Boyer J. S. Turgor and growth at low water potentials. Plant Physiol. 1989 Mar;89(3):798–804. doi: 10.1104/pp.89.3.798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Radin J. W., Boyer J. S. Control of Leaf Expansion by Nitrogen Nutrition in Sunflower Plants : ROLE OF HYDRAULIC CONDUCTIVITY AND TURGOR. Plant Physiol. 1982 Apr;69(4):771–775. doi: 10.1104/pp.69.4.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Silk W. K., Wagner K. K. Growth-sustaining Water Potential Distributions in the Primary Corn Root: A NONCOMPARTMENTED CONTINUUM MODEL. Plant Physiol. 1980 Nov;66(5):859–863. doi: 10.1104/pp.66.5.859. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Steudle E., Zimmermann U. Effect of turgor pressure and cell size on the wall elasticity of plant cells. Plant Physiol. 1977 Feb;59(2):285–289. doi: 10.1104/pp.59.2.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Twente J. W., Twente J. A. Regulation of hibernating periods by temperature. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1044–1051. [PMC free article] [PubMed] [Google Scholar]
  17. Van Volkenburgh E., Boyer J. S. Inhibitory effects of water deficit on maize leaf elongation. Plant Physiol. 1985 Jan;77(1):190–194. doi: 10.1104/pp.77.1.190. [DOI] [PMC free article] [PubMed] [Google Scholar]

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