Skip to main content
The Journal of Clinical Investigation logoLink to The Journal of Clinical Investigation
. 1958 Oct;37(10):1342–1347. doi: 10.1172/JCI103723

In Vitro Hydroxylation of Steroids by Whole Adrenal Homogenates of Beef, Normal Man, and Patients with the Adrenogenital Syndrome1

Alfred M Bongiovanni 1
PMCID: PMC1062808  PMID: 13575534

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTTER F. C., ALBRIGHT F., FORBES A. P., LEAF A., DEMPSEY E., CARROLL E. The effects of adrenocorticotropic hormone and cortisone in the adrenogenital syndrome associated with congenital adrenal hyperplasia: an attempt to explain and correct its disordered hormonal pattern. J Clin Invest. 1951 Mar;30(3):237–251. doi: 10.1172/JCI102438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BERLINER M. L., BERLINER D. L., DOUGHERTY T. F. Metabolism of progesterone by adrenal tissue from patients with Cushing's syndrome and mammary carcinoma. J Clin Endocrinol Metab. 1958 Jan;18(1):109–113. doi: 10.1210/jcem-18-1-109. [DOI] [PubMed] [Google Scholar]
  3. BONGIOVANNI A. M. Adrenogenital syndrome: uncomplicated and hypertensive forms. Pediatrics. 1958 Apr;21(4):661–667. [PubMed] [Google Scholar]
  4. BONGIOVANNI A. M., CLAYTON G. W., Jr A simplified method for the routine determination of pregnanediol and pregnanetriol in urine. Bull Johns Hopkins Hosp. 1954 Apr;94(4):180–186. [PubMed] [Google Scholar]
  5. BONGIOVANNI A. M., EBERLEIN W. R. Plasma and urinary corticosteroids in the hypertensive form of congenital adrenal hyperplasia. J Biol Chem. 1956 Nov;223(1):85–94. [PubMed] [Google Scholar]
  6. BONGIOVANNI A. M., EBERLEIN W. R., THOMAS P. Z. Use of an organic base in the Zimmermann reaction. J Clin Endocrinol Metab. 1957 Feb;17(2):331–332. doi: 10.1210/jcem-17-2-331. [DOI] [PubMed] [Google Scholar]
  7. BROWNIE A. C., GRANT J. K., DAVIDSON D. W. The in vitro enzymic hydroxylation of steroid hormones. II. Enzymic 11 beta-hydroxylation of progesterone by ox-adrenocortical mitochondria. Biochem J. 1954 Oct;58(2):218–225. doi: 10.1042/bj0580218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DORFMAN R. I., HAYANO M. The action of adrenal homogenates on progesterone, 17-hydroxyprogesterone and 21-desoxycortisone. Arch Biochem Biophys. 1952 Mar;36(1):237–239. doi: 10.1016/0003-9861(52)90397-4. [DOI] [PubMed] [Google Scholar]
  9. EBERLEIN W. R., BONGIOVANNI A. M. Congenital adrenal hyperplasia: an inborn error of metabolism. Helv Paediatr Acta. 1956 May;11(2):105–117. [PubMed] [Google Scholar]
  10. EBERLEIN W. R., BONGIOVANNI A. M. New solvent systems for the resolution of corticosteroids by paper chromatography. Arch Biochem Biophys. 1955 Nov;59(1):90–96. doi: 10.1016/0003-9861(55)90465-3. [DOI] [PubMed] [Google Scholar]
  11. EBERLEIN W. R., BONGIOVANNI A. M. Partial characterization of urinary adrenocortical steroids in adrenal hyperplasia. J Clin Invest. 1955 Aug;34(8):1337–1343. doi: 10.1172/JCI103181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ELY R. S., KELLEY V. C., RAILE R. B. Studies of 17-hydroxycorticosteroids in children. I. Peripheral blood levels in health and disease. J Pediatr. 1953 Jan;42(1):38–45. doi: 10.1016/s0022-3476(53)80107-1. [DOI] [PubMed] [Google Scholar]
  13. FUKUSHIMA D. K., GALLAGHER T. F. Steroid isolation studies in congenital adrenal hyperplasia. J Biol Chem. 1957 Nov;229(1):85–92. [PubMed] [Google Scholar]
  14. GORNALL A. G., MACDONALD M. P. Quantitative determination of the steroid hormones with 2, 4-dinitrophenylhydrazine. J Biol Chem. 1953 Mar;201(1):279–297. [PubMed] [Google Scholar]
  15. GRANT J. K., SYMINGTON T., DUGUID W. P. Effect of adrenocorticotropic therapy on the in vitro 11beta-hydroxylation of deoxycorticosterone by human adrenal homogenates. J Clin Endocrinol Metab. 1957 Aug;17(8):933–944. doi: 10.1210/jcem-17-8-933. [DOI] [PubMed] [Google Scholar]
  16. GRANT J. K. The in vitro enzymic hydroxylation of steroids. 4. The role of fumarate and triphosphopyridine nucleotide in the enzymic 11beta-hydroxylation of 11-deoxycorticosterone. Biochem J. 1956 Nov;64(3):559–567. doi: 10.1042/bj0640559. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. HAYANO M., DORFMAN R. I., PRINS D. A. Metabolism of the steroid hormone; conversion of desoxycorticosterone to glycogenic material in vitro. Proc Soc Exp Biol Med. 1949 Dec;72(3):700–700. doi: 10.3181/00379727-72-17547. [DOI] [PubMed] [Google Scholar]
  18. HAYANO M., DORFMAN R. I. The enzymatic C-11 beta-hydroxylation of steroids. J Biol Chem. 1953 Mar;201(1):175–188. [PubMed] [Google Scholar]
  19. HAYANO M., DORFMAN R. I., YAMADA E. Y. The conversion of desoxycorticosterone to glycogenic material by adrenal homogenates. J Biol Chem. 1951 Nov;193(1):175–181. [PubMed] [Google Scholar]
  20. HECHTER O., PINCUS G. Genesis of the adrenocortical secretion. Physiol Rev. 1954 Jul;34(3):459–496. doi: 10.1152/physrev.1954.34.3.459. [DOI] [PubMed] [Google Scholar]
  21. McGINTY D. A., SMITH G. N., WILSON M. L., WORREL C. S. The biosynthesis of 17-hydroxycorticosterone from 11-desoxy-17-hydroxy-corticosterone. Science. 1950 Oct 27;112(2913):506–506. doi: 10.1126/science.112.2913.506. [DOI] [PubMed] [Google Scholar]
  22. PLAGER J. E., SAMUELS L. T. The conversion of progesterone to 17-hydroxy-11-desoxycorticosterone by fractionated beef adrenal homogenates. J Biol Chem. 1954 Nov;211(1):21–29. [PubMed] [Google Scholar]
  23. PORTER C. C., SILBER R. H. A quantitative color reaction for cortisone and related 17,21-dihydroxy-20-ketosteroids. J Biol Chem. 1950 Jul;185(1):201–207. [PubMed] [Google Scholar]
  24. SAVARD K., GREEN A. A., LEWIS L. A. Oxidation of 11-desoxycorticosteroids with adrenal tissue homogenates. Endocrinology. 1950 Dec;47(6):418–428. doi: 10.1210/endo-47-6-418. [DOI] [PubMed] [Google Scholar]
  25. WILKINS L. The diagnosis of the adrenogenital syndrome and its treatment with cortisone. J Pediatr. 1952 Dec;41(6):860–874. doi: 10.1016/s0022-3476(52)80306-3. [DOI] [PubMed] [Google Scholar]
  26. WILSON H., FAIRBANKS R. Chromogenic values of 17-hydroxy corticosteroids in a modified Porter-Silber reaction. Arch Biochem Biophys. 1954 Nov;53(1):71–76. doi: 10.1016/0003-9861(54)90234-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation

RESOURCES