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. 2023 Oct 16;26(11):108219. doi: 10.1016/j.isci.2023.108219

Figure 3.

Figure 3

Differential effect of O-deglycosylation on CD13 antibody reactivity in human cancer CDX and normal tissues

(A) Western blot analyses and (B–D) quantified fold change of the reactivity of anti-CD13 antibodies on CD13 in human cancer CDX and normal tissue after O-deglycosylation in mAb 1–250 (B), mAb 400-50 (C), and mAb 687–967 (D), respectively. Mean fold change in protein band intensity was measured using Image Lab Software 6.1 and normalized to a β-actin loading control (E). HeatMap of anti-CD13 antibody reactivity to CD13 in human cancer CDX and normal tissues after O-glycosylation. Data shown are the mean of 3 independent experiments ±SEM. ∗∗p > 0.01, ∗∗∗p > 0.001 and ∗∗∗∗p > 0.0001 (two-way ANOVA).