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[Preprint]. 2023 Nov 1:2023.10.27.564454. [Version 1] doi: 10.1101/2023.10.27.564454

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Figure 1. — (A) A biological sample, such as patient blood, is analyzed using a mass or flow cytometer, which produces FCS data files. Manual gating is performed in FlowJo or Cytobank, creating a data file with specifications of each gate.

(B) Gate data and FCS files are imported into MetaGate, where a graphical user interface allows defining populations based on combinations of gates. Through a data reduction algorithm, a MetaGate data file is created, which contains marker expression and event frequencies of combinations of populations.

(C) The self-containing MetaGate data file is opened in the MetaGate graphical user interface for interactive production of statistics and plots, such as heatmaps, volcano plots and bar plots.