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[Preprint]. 2024 Jul 4:2023.11.05.565706. Originally published 2023 Nov 5. [Version 2] doi: 10.1101/2023.11.05.565706

Zinc stimulation of phytoplankton in a low carbon dioxide, coastal Antarctic environment

Riss M Kell, Adam V Subhas, Nicole L Schanke, Lauren E Lees, Rebecca J Chmiel, Deepa Rao, Margaret M Mars Brisbin, Dawn M Moran, Matthew R McIlvin, Francesco Bolinesi, Olga Mangoni, Raffaella Casotti, Cecilia Balestra, Tristan Horner, Robert B Dunbar, Andrew E Allen, Giacomo R DiTullio, Mak A Saito
PMCID: PMC10635156  PMID: 37961643

Abstract

Zinc (Zn) is a key micronutrient used by phytoplankton for carbon (C) acquisition, yet there have been few observations of its influence on natural oceanic phytoplankton populations. In this study, we observed Zn limitation of growth in the natural phytoplankton community of Terra Nova Bay, Antarctica, due to low (~220 μatm) pCO2 conditions, in addition to primary iron (Fe) limitation. Shipboard incubation experiments amended with Zn and Fe resulted in significantly higher chlorophyll a content and dissolved inorganic carbon drawdown compared to Fe addition alone. Zn and Fe response proteins detected in incubation and environmental biomass provided independent verification of algal co-stress for these micronutrients. These observations of Zn limitation under low pCO2 conditions demonstrate Zn can influence coastal primary productivity. Yet, as surface ocean pCO2 rises with continued anthropogenic emissions, the occurrence of Zn/C co-limitation will become rarer, impacting the biogeochemical cycling of Zn and other trace metal micronutrients.

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