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. 1984 Oct;76(2):531–535. doi: 10.1104/pp.76.2.531

Properties of Thylakoid Membranes of the Mangroves, Avicennia germinans and Avicennia marina, and the Sugar Beet, Beta vulgaris, Grown under Different Salinity Conditions 1

Marilyn C Ball 1,2, Scott E Taylor 1, Norman Terry 1
PMCID: PMC1064321  PMID: 16663875

Abstract

Properties of thylakoids isolated from leaves of three salt tolerant species, Avicennia germinans L., Avicennia marina var resinifera, and Beta vulgaris L., were not affected by the salinity in which the plants were grown. With increase in the growth salinity from 50 to 500 millimolar NaCl, there were no major effects on the per chlorophyll concentrations of lipids or proteins, or on the rates of uncoupled electron transport per chlorophyll mediated by either the whole chain or the partial reactions of photosystems I and II. Responses of the partial and whole chain reactions to variation in the sorbitol and NaCl concentrations in the assay media were independent of the salinity experienced during leaf growth and not substantially different from those of a salt-sensitive species, Cucurbita sativus L. Uncoupled rates of electron flow from water to p-benzoquinone mediated by photosystem II were insensitive to the NaCl concentration unless thylakoids were rendered Cl− deficient by treatment with uncoupler under alkaline conditions. Loss of 65% to 85% of the photosystem II activity in these Cl−-deficient thylakoids was restored by addition of 10 to 20 millimolar Cl−.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barber J. Stimulation of millisecond delayed light emission by KCl and NaCl gradients as a means of investigating the ionic permeability properties of the thylakoid membranes. Biochim Biophys Acta. 1972 Jul 12;275(1):105–116. doi: 10.1016/0005-2728(72)90029-1. [DOI] [PubMed] [Google Scholar]
  3. Delepelaire P., Chua N. H. Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci U S A. 1979 Jan;76(1):111–115. doi: 10.1073/pnas.76.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  5. Hind G., Nakatani H. Y., Izawa S. The role of Cl- in photosynthesis. I. The Cl- requirement of electron transport. Biochim Biophys Acta. 1969 Feb 25;172(2):277–289. doi: 10.1016/0005-2728(69)90070-x. [DOI] [PubMed] [Google Scholar]
  6. Larkum A. W., Hill A. E. Ion and water transport in Limonium. V. The ionic status of chloroplasts in the leaf of Limonium vulgare in relation to the activity of the salt glands. Biochim Biophys Acta. 1970 Mar 17;203(1):133–138. doi: 10.1016/0005-2736(70)90043-x. [DOI] [PubMed] [Google Scholar]
  7. Markwell M. A., Haas S. M., Tolbert N. E., Bieber L. L. Protein determination in membrane and lipoprotein samples: manual and automated procedures. Methods Enzymol. 1981;72:296–303. doi: 10.1016/s0076-6879(81)72018-4. [DOI] [PubMed] [Google Scholar]
  8. Pohl P., Glasl H., Wagner H. Zur Analytik pflanzlicher Glyko- und Phospholipoide und ihrer Fettsäuren. I. Eine neue dünnschichtchromatographische Methode zur Trennung pflanzlicher Lipoide und quantitativen Bestimmung ihrer Fettsäure-Zusammensetzung. J Chromatogr. 1970 Jun 24;49(3):488–492. doi: 10.1016/s0021-9673(00)93664-1. [DOI] [PubMed] [Google Scholar]
  9. Robinson S. P., Downton W. J., Millhouse J. A. Photosynthesis and ion content of leaves and isolated chloroplasts of salt-stressed spinach. Plant Physiol. 1983 Oct;73(2):238–242. doi: 10.1104/pp.73.2.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Robinson S. P., Downton W. J. Potassium, sodium, and chloride content of isolated intact chloroplasts in relation to ionic compartmentation in leaves. Arch Biochem Biophys. 1984 Jan;228(1):197–206. doi: 10.1016/0003-9861(84)90061-4. [DOI] [PubMed] [Google Scholar]
  11. Roughan P. G., Batt R. D. Quantitative analysis of sulfolipid (sulfoquinovosyl diglyceride) and galactolipids (monogalactosyl and digalactosyl diglycerides) in plant tissues. Anal Biochem. 1968 Jan;22(1):74–88. doi: 10.1016/0003-2697(68)90261-3. [DOI] [PubMed] [Google Scholar]
  12. Stewart A. C. The effects of high concentrations of salts on photosynthetic electron transport in spinach (Spinacia oleracea) chloroplasts. Biochem J. 1982 Jun 15;204(3):705–712. doi: 10.1042/bj2040705. [DOI] [PMC free article] [PubMed] [Google Scholar]

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