Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1984 Nov;76(3):846–848. doi: 10.1104/pp.76.3.846

Disulfiram Metabolism in Isolated Mesophyll Cells and Inhibition of Photosynthesis and Cyanide-Resistant Respiration 1

Alan W Bown 1, John Pullen 1, Nancy M Shadeed 1
PMCID: PMC1064387  PMID: 16663938

Abstract

Tetraethylthiuram disulfide (disulfiram) stimulated medium acidification when added at a concentration of 0.4 millimolar to illuminated or nonilluminated suspensions of Asparagus sprengeri Regel mesophyll cells. Similar concentrations inhibited photosynthesis and cyanide-resistant respiration. The reduction product of disulfiram, diethyldithiocarbamic acid, accumulated in concentrations sufficient to account for the observed acidification.

Full text

PDF
846

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnon D. I. COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS. Plant Physiol. 1949 Jan;24(1):1–15. doi: 10.1104/pp.24.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bown A. W. An investigation into the roles of photosynthesis and respiration in h efflux from aerated suspensions of asparagus mesophyll cells. Plant Physiol. 1982 Sep;70(3):803–810. doi: 10.1104/pp.70.3.803. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Duhaime D. E., Bown A. W. Stimulation of h efflux and inhibition of photosynthesis by esters of carboxylic acids. Plant Physiol. 1983 Nov;73(3):828–833. doi: 10.1104/pp.73.3.828. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Grover S. D., Laties G. G. Disulfiram inhibition of the alternative respiratory pathway in plant mitochondria. Plant Physiol. 1981 Aug;68(2):393–400. doi: 10.1104/pp.68.2.393. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. JOHNSTON C. D. The in vitro reaction between tetraethylthiuram disulfide (antabuse) and glutathione. Arch Biochem Biophys. 1953 May;44(1):249–251. doi: 10.1016/0003-9861(53)90034-4. [DOI] [PubMed] [Google Scholar]
  6. Miller M. G., Obendorf R. L. Use of Tetraethylthiuram Disulfide to Discriminate between Alternative Respiration and Lipoxygenase. Plant Physiol. 1981 May;67(5):962–964. doi: 10.1104/pp.67.5.962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Neims A. H., Coffey D. S., Hellerman L. Interaction between tetraethylthiuram disulfide and the sulfhydryl groups of D-amino acid oxidase and of hemoglobin. J Biol Chem. 1966 Dec 25;241(24):5941–5948. [PubMed] [Google Scholar]
  8. SIJPESTEIJN A. K., KASLANDER J., van der KERK G. On the conversion of sodium dimethyldithiocarbamate into its alpha-aminobutyric acid derivative by microorganisms. Biochim Biophys Acta. 1962 Aug 27;62:587–589. doi: 10.1016/0006-3002(62)90245-7. [DOI] [PubMed] [Google Scholar]
  9. Werdan K., Heldt H. W., Milovancev M. The role of pH in the regulation of carbon fixation in the chloroplast stroma. Studies on CO2 fixation in the light and dark. Biochim Biophys Acta. 1975 Aug 11;396(2):276–292. doi: 10.1016/0005-2728(75)90041-9. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES