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. 1984 Dec;76(4):943–946. doi: 10.1104/pp.76.4.943

Depolarization of Cell Membrane Potential during Trans-Plasma Membrane Electron Transfer to Extracellular Electron Acceptors in Iron-Deficient Roots of Phaseolus vulgaris L. 1

Peter C Sijmons 1,2, Frank C Lanfermeijer 1,2, Albertus H de Boer 1,2, Hidde B A Prins 1,2, H Frits Bienfait 1,2
PMCID: PMC1064411  PMID: 16663976

Abstract

Transfer of electrons from the cytosol of bean (Phaseolus vulgaris L.) root cells to extracellular acceptors such as ferricyanide and FeIIIEDTA causes a rapid depolarization of the membrane potential. This effect is most pronounced (30-40 millivolts) with root cells of Fe-deficient plants, which have an increased capacity to reduce extracellular ferric salts. Ferrocyanide has no effect. In the state of ferricyanide reduction, H+ (1H+/2 electrons) and K+ ions are excreted. The reduction of extracellular ferric salts by roots of Fe-deficient bean plants is driven by cellular NADPH (Sijmons, van den Briel, Bienfait 1984 Plant Physiol 75: 219-221). From this and from the membrane potential depolarization, we conclude that trans-plasma membrane electron transfer from NADPH is the primary process in the reduction of extracellular ferric salts.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ambler J. E., Brown J. C. Iron-stress Response in Mixed and Monocultures of Soybean Cultivars. Plant Physiol. 1972 Dec;50(6):675–678. doi: 10.1104/pp.50.6.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson W. P., Hendrix D. L., Higinbotham N. Higher plant cell membrane resistance by a single intracellular electrode method. Plant Physiol. 1974 Jan;53(1):122–124. doi: 10.1104/pp.53.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barrett-Lennard E. G., Marschner H., Römheld V. Mechanism of Short Term Fe Reduction by Roots : Evidence against the Role of Secreted Reductants. Plant Physiol. 1983 Dec;73(4):893–898. doi: 10.1104/pp.73.4.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bunting P. S., Laidler K. J. Flow kinetics of L-asparaginase attached to nylon tubing. Biotechnol Bioeng. 1974 Jan;16(1):119–134. doi: 10.1002/bit.260160109. [DOI] [PubMed] [Google Scholar]
  5. Chaney R. L., Brown J. C., Tiffin L. O. Obligatory reduction of ferric chelates in iron uptake by soybeans. Plant Physiol. 1972 Aug;50(2):208–213. doi: 10.1104/pp.50.2.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Crane F. L., Roberts H., Linnane A. W., Löw H. Transmembrane ferricyanide reduction by cells of the yeast Saccharomyces cerevisiae. J Bioenerg Biomembr. 1982 Jun;14(3):191–205. doi: 10.1007/BF00745020. [DOI] [PubMed] [Google Scholar]
  7. Federico R., Giartosio C. E. A transplasmamembrane electron transport system in maize roots. Plant Physiol. 1983 Sep;73(1):182–184. doi: 10.1104/pp.73.1.182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lin W. Further Characterization on the Transport Property of Plasmalemma NADH Oxidation System in Isolated Corn Root Protoplasts. Plant Physiol. 1984 Feb;74(2):219–222. doi: 10.1104/pp.74.2.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Römheld V., Marschner H. Mechanism of iron uptake by peanut plants : I. Fe reduction, chelate splitting, and release of phenolics. Plant Physiol. 1983 Apr;71(4):949–954. doi: 10.1104/pp.71.4.949. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sijmons P. C., van den Briel W., Bienfait H. F. Cytosolic NADPH is the electron donor for extracellular fe reduction in iron-deficient bean roots. Plant Physiol. 1984 May;75(1):219–221. doi: 10.1104/pp.75.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]

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