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. 1984 Dec;76(4):1070–1078. doi: 10.1104/pp.76.4.1070

Intracellular Localization of Lipoxygenases-1 and -2 in Germinating Soybean Seeds by Indirect Labeling with Protein A-Colloidal Gold Complexes

Marjan Vernooy-Gerritsen 1,2, Jan L M Leunissen 1,2, Gerrit A Veldink 1,2, Johannes F G Vliegenthart 1,2
PMCID: PMC1064436  PMID: 16663951

Abstract

Soybean lipoxygenases-1 and -2 were localized intracellularly in seeds at various stages of germination by indirect labeling of cryosections with protein A-colloidal gold complexes. Two sizes of gold particles (Au5 and Au16) were used in single- and double-labeling experiments. In primary leaves, lipoxygenases are demonstrated to occur in vacuolating parenchyma cells but not in massive, nondifferentiated cells. In cotyledons, both isoenzymes are localized in the cytoplasm of storage parenchyma cells and in an aberrant type of protein bodies, occurring in hypodermis and vascular bundle sheath cells. No association has been found with either protein bodies in storage parenchyma cells or lipid bodies, mitochondria, and other organelles in any type of cell. The possible significance of lipoxygenase in the metabolism of storage lipids and its possible function as a regulatory enzyme are discussed on the basis of the random distribution throughout the cytoplasm of storage parenchyma cells and the course of biochemical processes during seed germination.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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