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. 1985 Jan;77(1):99–103. doi: 10.1104/pp.77.1.99

Cytokinin-Modulated Gene Expression in Excised Pumpkin Cotyledons 1

Chong-Maw Chen 1, Scott M Leisner 1
PMCID: PMC1064464  PMID: 16664037

Abstract

Comparison of two-dimensional polyacrylamide gel electrophoretic maps of proteins isolated from benzyladenine-treated and untreated pumpkin (Cucurbita pepo L. cv Halloween) cotyledons showed that the expression of certain proteins is enhanced, induced, or suppressed by the cytokinin treatment. The amount of poly(A)+ mRNA isolated from cotyledons incubated with 10−4 molar benzyladenine for five days was about four-fold over the water-incubated control. The activity of hydroxypyruvate reductase prepared from purified cotyledonous microbodies and analyzed by native gel electrophoresis is proportionally enhanced by sequentially higher concentrations (10−9 to 10−4 molar) of benzyladenine. Ethidium bromide (1 microgram per milliliter) did not inhibit hydroxypyruvate reductase activity; thus, the enzyme synthesis does not appear to be controlled by organelle genes. Hydroxypyruvate reductase synthesis is inhibited by cycloheximide, cordycepin, and to a certain degree by actinomycin D. These data support the view of a close association between cytokinin action and gene expression.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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