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. 1985 Jan;77(1):229–231. doi: 10.1104/pp.77.1.229

Effect of Ferredoxin on the Diaphorase Activity of Cyanobacterial Ferredoxin-NADP Reductase 1

H Melamed-Harel 1,2, Elisha Tel-Or 1,2, Anthony San Pietro 1,2
PMCID: PMC1064488  PMID: 16664015

Abstract

The interaction of ferredoxin-NADP reductase (FNR) and ferredoxin (Fd) results in an enhanced rate of reaction and a shift of the pH optimum for the FNR-mediated diaphorase reaction. Low concentrations of NaCl (<100 millimolar), favorable for formation of the FNR:Fd complex, further magnify the alteration of the diaphorase reaction; the activity is enhanced 3-fold and pH optimum is shifted from 9.5 to 7.8. The Fd-stimulated diaphorase activity of FNR may result either from a conformational change of the enzyme and/or from a transition from a two electron to a one electron reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AVRON M., JAGENDORF A. T. A TPNH diaphorase from chloroplasts. Arch Biochem Biophys. 1956 Dec;65(2):475–490. doi: 10.1016/0003-9861(56)90207-7. [DOI] [PubMed] [Google Scholar]
  2. Allen M. B., Arnon D. I. Studies on Nitrogen-Fixing Blue-Green Algae. I. Growth and Nitrogen Fixation by Anabaena Cylindrica Lemm. Plant Physiol. 1955 Jul;30(4):366–372. doi: 10.1104/pp.30.4.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bookjans G., San Pietro A., Böger P. Resolution and reconstitution of spinach ferredoxin-NADP+ reductase. Biochem Biophys Res Commun. 1978 Feb 28;80(4):759–765. doi: 10.1016/0006-291x(78)91310-4. [DOI] [PubMed] [Google Scholar]
  4. Davis D. J., Pietro A. S. Chemical modification of spinach ferredoxin: evidence for the involvement of a complex between ferredoxin and ferredoxin:NADP oxidoreductase in NADP photoreduction. Biochem Biophys Res Commun. 1977 Jan 10;74(1):33–40. doi: 10.1016/0006-291x(77)91371-7. [DOI] [PubMed] [Google Scholar]
  5. Davis D. J., San Pietro A. Evidence for the role of sulfhydryl groups in a pH-dependent transition of ferredoxin:NADP oxidoreductase. Arch Biochem Biophys. 1977 Dec;184(2):572–577. doi: 10.1016/0003-9861(77)90467-2. [DOI] [PubMed] [Google Scholar]
  6. Foust G. P., Mayhew S. G., Massey V. Complex formation between ferredoxin triphosphopyridine nucleotide reductase and electron transfer proteins. J Biol Chem. 1969 Feb 10;244(3):964–970. [PubMed] [Google Scholar]
  7. Hall D. O., Rao K. K., Cammack R. A stable and easily extractable plant-type ferredoxin from the blue-green alga Spirulina maxima. Biochem Biophys Res Commun. 1972 May 26;47(4):798–802. doi: 10.1016/0006-291x(72)90562-1. [DOI] [PubMed] [Google Scholar]
  8. Keirns J. J., Wang J. H. Studies on nicotinamide adenine dinucleotide phosphate reductase of spinach chloroplasts. J Biol Chem. 1972 Nov 25;247(22):7374–7382. [PubMed] [Google Scholar]
  9. LAZZARINI R. A., SAN PIETRO A. The reduction of cytochrome c by photosynthetic pyridine nucleotide reductase and transhydrogenase. Biochim Biophys Acta. 1962 Aug 13;62:417–420. doi: 10.1016/0006-3002(62)90273-1. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Masaki R., Wada K., Matsubara H. Isolation and characterization of two ferredoxin-NADP+ reductases from Spirulina platensis. J Biochem. 1979 Oct;86(4):951–962. doi: 10.1093/oxfordjournals.jbchem.a132627. [DOI] [PubMed] [Google Scholar]
  12. Nakamura S., Kimura T. Studies on spinach ferredoxin-nicotinamide adenine dinucleotide phosphate reductase. Kinetic studies on the interactions of the reductase and ferredoxin and a possible regulation of enzyme activities by ionic strength. J Biol Chem. 1971 Oct 25;246(20):6235–6241. [PubMed] [Google Scholar]
  13. Nelson N., Neumann J. Interaction between ferredoxin and ferredoxin nicotinamide adenine dinucleotide phosphate reductase in pyridine nucleotide photoreduction and some partial reactions. I. Inhibition of ferredoxin nicotinamide adenine dinucleotide phosphate reductase by ferredoxin. J Biol Chem. 1969 Apr 10;244(7):1926–1931. [PubMed] [Google Scholar]
  14. Nelson N., Neumann J. Interaction between ferredoxin and ferredoxin nicotinamide adenine dinucleotide phosphate reductase in pyridine nucleotide photoreduction and some partial reactions. II. Complex formation between ferredoxin and ferredoxin nicotinamide adenine dinucleotide phosphate reductase and its relevance to pyridine nucleotide photoreduction. J Biol Chem. 1969 Apr 10;244(7):1932–1936. [PubMed] [Google Scholar]
  15. SHIN M., ARNON D. I. ENZYMIC MECHANISMS OF PYRIDINE NUCLEOTIDE REDUCTION IN CHLOROPLASTS. J Biol Chem. 1965 Mar;240:1405–1411. [PubMed] [Google Scholar]
  16. Shin M., Pietro A. S. Complex formation of ferredoxin-NADP reductase with ferredoxin and with NADP. Biochem Biophys Res Commun. 1968 Oct 10;33(1):38–42. doi: 10.1016/0006-291x(68)90251-9. [DOI] [PubMed] [Google Scholar]
  17. Zanetti G., Forti G. Studies on the triphosphopyridine nucleotide-cytochrome f reductase of chloroplasts. J Biol Chem. 1966 Jan 25;241(2):279–285. [PubMed] [Google Scholar]

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