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. 1985 Feb;77(2):335–338. doi: 10.1104/pp.77.2.335

Effect of Temperature on H2 Evolution and Acetylene Reduction in Pea Nodules and in Isolated Bacteroids

Hans Bertelsen 1
PMCID: PMC1064515  PMID: 16664054

Abstract

Nitrogenase (EC 1.7.99.2) activity in pea (Pisum savitum) nodules formed after infection with Rhizobium leguminosarum (lacking uptake hydrogenase) was measured as acetylene reduction, H2 evolution in air and H2 evolution in Ar:O2. With detached roots the relative efficiency, calculated from acetylene reduction, showed a decrease (from 55 to below 0%) with increasing temperature. With excised nodules and isolated bacteroids similar results were obtained. However, the relative efficiency calculated from H2 evolution in Ar:O2 was unaffected by temperature. Measurements on both excised nodules and isolated bacteroids showed a marked difference between acetylene reduction and H2 evolution in Ar:O2 with increased temperature, indicating that either acetylene reduction or H2 evolution in Ar:O2 are inadequate measures of nitrogenase activity at higher temperature.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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