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. 1985 Feb;77(2):379–381. doi: 10.1104/pp.77.2.379

Regulation of NO3− Influx in Barley 1

Studies Using 13NO3−

Anthony D M Glass 1,2,3, Robert G Thompson 1,2,3, Lucien Bordeleau 1,2,3
PMCID: PMC1064523  PMID: 16664062

Abstract

Short-term (10 minutes) measurements of plasmalemma NO3− influx (φoc) into roots of intact barley plants were obtained using 13NO3−. In plants grown for 4 days at various NO3− levels (0.1, 0.2, 0.5 millimolar), φoc was found to be independent of the level of NO3− pretreatment. Similarly, pretreatment with Cl− had no effect upon plasmalemma 13NO3− influx. Plants grown in the complete absence of 13NO3− (in CaSO4 solutions) subsequently revealed influx values which were more than 50% lower than for plants grown in NO3−. Based upon the documented effects of NO3− or Cl− pretreatments on net uptake of NO3−, these observations suggest that negative feedback from vacuolar NO3− and/or Cl− acts at the tonoplast but not at the plasmalemma. When included in the influx medium, 0.5 millimolar Cl− was without effect upon 13NO3− influx, but NH4+ caused approximately 50% reduction of influx at this concentration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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