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. 1985 Mar;77(3):587–590. doi: 10.1104/pp.77.3.587

Protein Metabolism in Senescing Wheat Leaves 1

Determination of Synthesis and Degradation Rates and Their Effects on Protein Loss

Lorenzo Lamattina 1,2, Rafael Pont Lezica 1,2, Rubén D Conde 1,2
PMCID: PMC1064569  PMID: 16664103

Abstract

Wheat leaves (Triticum aestivum L.) at the moment of their maximum expansion were detached and put in darkness. Their protein, RNA and DNA contents, as well as their rates of protein synthesis and degradation, were measured at different times from 0 to 5 days after detachment. Rates of protein synthesis were measured by incorporation into proteins of large amounts of [3H]leucine. Fractional rates of protein degradation were estimated either from the difference between the rates of synthesis and the net protein change or by the disappearance of radioactivity from proteins previously labeled with [3H]leucine or [14C]proline.

Protein loss reached a value of 20% during the first 48 hours of the process. RNA loss paralleled that of protein, whereas DNA content proved to be almost constant during the first 3 days and decreased dramatically thereafter.

Measurements of protein synthesis and degradation indicate that, in spite of a slowdown in rate of protein synthesis, an increased rate of protein breakdown is mainly responsible for the observed rapid protein loss.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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