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. 1985 Apr;77(4):791–794. doi: 10.1104/pp.77.4.791

Characterization of Glutamine Synthetase Isoforms from Chlorella1

Rob F Beudeker 1,2,2, F Robert Tabita 1,2
PMCID: PMC1064607  PMID: 16664139

Abstract

Ion-exchange chromatography of extracts derived from Chlorella sorokiniana mutant strain (oxygen resistant) yielded two separate activity peaks of glutamine synthetase (GS). GSI and GSII were purified 220- and 187-fold and have molecular weights of approximately 398,000 and 360,000, respectively. Both enzymes are composed of eight identical subunits with a subunit molecular weight of 47,000 for GSI and 43,000 for GSII. The amino acid composition, catalytic, and immunological properties for both enzymes are similar.

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Selected References

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