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. 1985 Apr;77(4):896–902. doi: 10.1104/pp.77.4.896

Naringin Levels in Citrus Tissues 1

I. Comparison of Different Antibodies and Tracers for the Radioimmunossay of Naringin

Pablo S Jourdan 1,2, Elmar W Weiler 1,2, Richard L Mansell 1,2
PMCID: PMC1064627  PMID: 16664158

Abstract

The preparation of a tritiated radiotracer that was used in the radioimmunoassay of naringin (naringenin-7-O-α-rhamnosyl- (1-2)-β-d-glucopyranoside) and which was synthesized by reduction of the carbonyl group of the flavanone is reported. The resulting assay has a detection limit of 0.5 picomole per 0.1 milliliter, is specific for the 7-neohesperidoside substitution on flavanones, and can measure naringin in crude extracts of plant tissues. This radioimmunoassay is compared with three other naringin immunoassays which use antibodies raised against two different haptens and different tracers labeled with 125I or 3H. The applicability of the methods to the quantification of naringin and other flavanone neohesperidosides in citrus tissue is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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