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. 1985 Apr;77(4):1010–1012. doi: 10.1104/pp.77.4.1010

Isolation of Mitochondria from Soybean Leaves on Discontinuous Percoll Gradients 1

Terry C Hrubec 1,2, J Michael Robinson 1,2, Robert P Donaldson 1,2
PMCID: PMC1064649  PMID: 16664137

Abstract

A technique to isolate mitochondria from chamber-grown soybeans (Glycine max cv Williams) was developed. The mitochondria were isolated by centrifugation on discontinuous Percoll gradients which yielded a sharp band of mitochondria contaminated by only 4% of the total chlorophyll in the gradient. Contamination by peroxisomes was also slight. The isolated mitochondria oxidized malate plus glutamate, NADH, and malate with respiratory control. They also showed cyanide-insensitive, alternative pathway activity which was inhibited by salicylhydroxamic acid.

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Selected References

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