Abstract
A small quantity of (1→3)-β-d-glucan was extracted with a (1→3),(1→4)-β-d-glucan by hot water after treatment of the insoluble fraction of a buffer homogenate of Zea shoots with 3 molar LiCl. An ammonium sulfate precipitation procedure effected a separation of the (1→3)-β-d-glucan from the more prevalent (1→3),(1→4)-β-d-glucan. The minor component polysaccharide precipitated at a concentration of 20% ammonium sulfate (w/v) and was, as a consequence of precipitation, rendered insoluble in water. The insoluble products were dissolved in 1 normal NaOH followed by neutralization with CH3COOH. The purified polysaccharide accounted for approximately 0.3% of total hot water extract. It consisted mostly of glucose and its average mol wt was estimated to be about 7.0 × 104, based on elution from a calibrated Sepharose CL-4B column. Methylation analysis and enzymic hydrolysis or partial acid-hydrolysis of the polysaccharide followed by analysis of the hydrolysate showed that the polysaccharide consisted of (1→3)-β-linked glucose residues.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- HAKOMORI S. A RAPID PERMETHYLATION OF GLYCOLIPID, AND POLYSACCHARIDE CATALYZED BY METHYLSULFINYL CARBANION IN DIMETHYL SULFOXIDE. J Biochem. 1964 Feb;55:205–208. [PubMed] [Google Scholar]
- Kato Y., Nevins D. J. Enzymic Dissociation of Zea Shoot Cell Wall Polysaccharides : II. Dissociation of (1 --> 3),(1 --> 4)-beta-d-Glucan by Purified (1 --> 3),(1 --> 4)-beta-d-Glucan 4-Glucanohydrolase from Bacillus subtilis. Plant Physiol. 1984 Jul;75(3):745–752. doi: 10.1104/pp.75.3.745. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kato Y., Nevins D. J. Enzymic dissociation of zea shoot cell wall polysaccharides : I. Preliminary characterization of the water-insoluble fraction of zea shoot cell walls. Plant Physiol. 1984 Jul;75(3):740–744. doi: 10.1104/pp.75.3.740. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kato Y., Nevins D. J. Structure of the arabinogalactan from zea shoots. Plant Physiol. 1984 Mar;74(3):562–568. doi: 10.1104/pp.74.3.562. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maltby D., Carpita N. C., Montezinos D., Kulow C., Delmer D. P. beta-1,3-Glucan in Developing Cotton Fibers: Structure, Localization, and Relationship of Synthesis to That of Secondary Wall Cellulose. Plant Physiol. 1979 Jun;63(6):1158–1164. doi: 10.1104/pp.63.6.1158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROBYT J., FRENCH D. Action pattern and specificity of an amylase from Bacillus subtilis. Arch Biochem Biophys. 1963 Mar;100:451–467. doi: 10.1016/0003-9861(63)90112-7. [DOI] [PubMed] [Google Scholar]
- Sandford P. A., Conrad H. E. The structure of the Aerobacter aerogenes A3(S1) polysaccharide. I. A reexamination using improved procedures for methylation analysis. Biochemistry. 1966 May;5(5):1508–1517. doi: 10.1021/bi00869a009. [DOI] [PubMed] [Google Scholar]