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. 1985 May;78(1):41–45. doi: 10.1104/pp.78.1.41

Energetics of Sucrose Transport into Protoplasts from Developing Soybean Cotyledons 1

Willy Lin 1
PMCID: PMC1064672  PMID: 16664205

Abstract

The accumulation of tetraphenylphosphonium (TPP+), 5,5′-dimethyl-oxazolidine-2,4-dione (DMO), and a micro pH electrode were used to measure membrane potential, intracellular and extracellular pH, respectively, upon the addition of exogenous sucrose to soybean cotyledon protoplasts. Addition of sucrose caused a specific and transient (a) depolarization of the membrane potential (measured by TPP+ accumulation), (b) acidification of the intracellular pH (measured by DMO accumulation), and (c) alkalization of the external medium (measured by a micro pH electrode). The time course for all these changes was similar (i.e. 5 to 10 minutes). Based on the rate of sucrose uptake and alkalization of the external medium, a stoichiometry of 1.02 to 1.10 for proton to sucrose was estimated. These data strongly support a proton/sucrose cotransporting mechanism in soybean cotyledon cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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