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. 1985 May;78(1):163–167. doi: 10.1104/pp.78.1.163

Na+/H+ Antiport in Isolated Tonoplast Vesicles from Storage Tissue of Beta vulgaris1

Eduardo Blumwald 1, Ronald J Poole 1
PMCID: PMC1064695  PMID: 16664191

Abstract

The pH-dependent fluorescence quenching of acridine orange was used to study the Na+- and K+-dependent H+ fluxes in tonoplast vesicles isolated from storage tissue of red beet and sugar beet (Beta vulgaris L.). The Na+-dependent H+ flux across the tonoplast membrane could be resolved into two components: (a) a membrane potential-mediated flux through conductive pathways; and (b) an electroneutral flux which showed Michaelis-Menten kinetics relationship to Na+ concentration and was competitively inhibited by amiloride (Ki = 0.1 millimolar). The potential-dependent component of H+ flux showed an approximately linear dependence on Na+ concentration. In contrast, the K+-dependent H+ flux apparently consisted of a single component which showed an approximately linear dependence on K+ concentration, and was insensitive to amiloride. Based on the Na+- and K+-dependent H+ fluxes, the passive permeability of the vesicle preparation to Na+ was about half of that to K+.

The apparent Km for Na+ of the electroneutral Na+/H+ exchange varied by more than 3-fold (7.5-26.5 millimolar) when the internal and external pH values were changed in parallel. The results suggest a simple kinetic model for the operation of the Na+/H+ antiport which can account for the estimated in vivo accumulation ratio for Na+ into the vacuole.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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