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. 1985 May;78(1):178–182. doi: 10.1104/pp.78.1.178

Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis

Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction

Catherine Colas des Francs 1, Hervé Thiellement 1, Dominique de Vienne 1
PMCID: PMC1064698  PMID: 16664194

Abstract

A previous genetic analysis by two-dimensional electrophoresis of wheat leaf proteins led us to hypothesize that several polypeptides were degradation products of the large subunit of ribulose bisphosphate carboxylase/oxygenase (EC 4.1.1.39).

Various extraction procedures including the use of proteinase inhibitors allowed us to: (a) confirm that the suspected polypeptides are indeed degradation products; (b) find out under which conditions the proteinases act; (c) find a method which prevents degradation, by boiling the extract in the presence of sodium dodecyl sulfate and 2-mercaptoethanol.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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