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. 1985 Jun;78(2):232–240. doi: 10.1104/pp.78.2.232

Evidence for an ATP-Dependent Proton Pump on the Golgi of Corn Coleoptiles 1

Alain Chanson 1,2, Lincoln Taiz 1
PMCID: PMC1064711  PMID: 16664222

Abstract

Corn (Zea mays L. cv Trojan T929) coleoptile membranes were fractionated on sucrose density gradients, and ATP-dependent proton pumping activity was localized by the techniques of [14C]methylamine uptake and quinacrine fluorescence quenching. Two peaks of proton pumping activity were detected: a light peak (1.07 grams/cubic centimeter) corresponding to the previously characterized tonoplast-type H+-ATPase, and a second peak (1.13 grams/cubic centimeter) which coincided with the Golgi markers, latent UDPase, and glucan synthase I. The second peak was lighter than that of the plasma membrane marker, uridine diphosphoglucose-sterol glucosyltransferase (1.16 grams/cubic centimeter) and was not inhibited by vanadate, an inhibitor of the plasma membrane ATPase. The activity was also better correlated with the Golgi cisternae marker, glucan synthase I, than with latent UDPase, a secretory vesicle marker, but a secretory vesicle location cannot be ruled out. The tonoplast-type and Golgi proton pumps were similar in several respects, including a pH optimum at 7.2, stimulation by chloride, inhibition by diethylstilbestrol and N,N′-dicyclohexylcarbodiimide (DCCD), insensitivity to oligomycin and azide, and nucleotide specificity for Mg2+-ATP. However, the Golgi H+ pump was much less sensitive to nitrate and iodide, and more sensitive to the anion channel blockers, 4-acetamido-4′-isothiocyano-2,2′-stilbene sulfonic acid (SITS) and 4,4′-diisothiocyano-2,2′-stilbene disulfonic acid (DIDS) than the tonoplast-type H+-pump. The Golgi pump, but not the tonoplast-type pump, was stimulated by valinomycin in the presence of KCl. It is concluded that the Golgi of corn coleoptiles contains a KCl-stimulated H+-ATPase which can acidify the interior of Golgi cisternae and associated vesicles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barr R., Safranski K., Sun I. L., Crane F. L., Morré D. J. An electrogenic proton pump associated with the Golgi apparatus of mouse liver driven by NADH and ATP. J Biol Chem. 1984 Nov 25;259(22):14064–14067. [PubMed] [Google Scholar]
  2. Binari L. L., Racusen R. H. Membrane-associated ATPases in isolated secretory vesicles. Plant Physiol. 1983 Mar;71(3):594–597. doi: 10.1104/pp.71.3.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Briskin D. P., Leonard R. T. Isolation of tonoplast vesicles from tobacco protoplasts. Plant Physiol. 1980 Oct;66(4):684–687. doi: 10.1104/pp.66.4.684. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chanson A., McNaughton E., Taiz L. Evidence for a KCl-Stimulated, Mg-ATPase on the Golgi of Corn Coleoptiles. Plant Physiol. 1984 Oct;76(2):498–507. doi: 10.1104/pp.76.2.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Glickman J., Croen K., Kelly S., Al-Awqati Q. Golgi membranes contain an electrogenic H+ pump in parallel to a chloride conductance. J Cell Biol. 1983 Oct;97(4):1303–1308. doi: 10.1083/jcb.97.4.1303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Higgins J. A. The transverse distribution of phospholipids in the membranes of Golgi subfractions of rat hepatocytes. Biochem J. 1984 Apr 1;219(1):261–272. doi: 10.1042/bj2190261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mandala S., Mettler I. J., Taiz L. Localization of the proton pump of corn coleoptile microsomal membranes by density gradient centrifugation. Plant Physiol. 1982 Dec;70(6):1743–1747. doi: 10.1104/pp.70.6.1743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mettler I. J., Mandala S., Taiz L. Characterization of in vitro proton pumping by microsomal vesicles isolated from corn coleoptiles. Plant Physiol. 1982 Dec;70(6):1738–1742. doi: 10.1104/pp.70.6.1738. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Orci L., Montesano R., Meda P., Malaisse-Lagae F., Brown D., Perrelet A., Vassalli P. Heterogeneous distribution of filipin--cholesterol complexes across the cisternae of the Golgi apparatus. Proc Natl Acad Sci U S A. 1981 Jan;78(1):293–297. doi: 10.1073/pnas.78.1.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Poole R. J., Briskin D. P., Krátký Z., Johnstone R. M. Density gradient localization of plasma membrane and tonoplast from storage tissue of growing and dormant red beet : characterization of proton-transport and ATPase in tonoplast vesicles. Plant Physiol. 1984 Mar;74(3):549–556. doi: 10.1104/pp.74.3.549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ray P. M. Auxin-binding Sites of Maize Coleoptiles Are Localized on Membranes of the Endoplasmic Reticulum. Plant Physiol. 1977 Apr;59(4):594–599. doi: 10.1104/pp.59.4.594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Vara F., Serrano R. Partial purification and properties of the proton-translocating ATPase of plant plasma membranes. J Biol Chem. 1982 Nov 10;257(21):12826–12830. [PubMed] [Google Scholar]
  13. West D. W., Clegg R. A. Transient formation of the phosphoprotein during autophosphorylation of rat mammary gland Golgi vesicles. Biochim Biophys Acta. 1982 Sep 9;690(2):290–295. doi: 10.1016/0005-2736(82)90333-9. [DOI] [PubMed] [Google Scholar]
  14. Zhang F., Schneider D. L. The bioenergetics of Golgi apparatus function: evidence for an ATP-dependent proton pump. Biochem Biophys Res Commun. 1983 Jul 29;114(2):620–625. doi: 10.1016/0006-291x(83)90825-2. [DOI] [PubMed] [Google Scholar]

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