Skip to main content
PMC home page
Plant Physiology logoLink to Plant Physiology
. 1985 Jul;78(3):438–441. doi: 10.1104/pp.78.3.438

Tyrosine and Phenylalanine Ammonia Lyase Activities during Shoot Initiation in Tobacco Callus Cultures 1

Lynda D Beaudoin-Eagan 1, Trevor A Thorpe 1
PMCID: PMC1064755  PMID: 16664262

Abstract

Both phenylalanine ammonia lyase and tyrosine ammonia lyase were detected in tobacco (Nicotiana tabacum L. Wisconsin 38) callus. The enzymes were separated from each other by Sephadex G-200 column chromatography. Increased activity of tyrosine ammonia lyase was observed during culture of tobacco callus under shoot-forming conditions, while activity of phenylalanine ammonia lyase increased during culture under non-organ-forming conditions. Confirmation of these findings was obtained by examining the incorporation of [14C]tyrosine and [14C]phenylalanine into p-coumarate and trans-cinnamate, respectively.

Full text

PDF
438

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Beaudoin-Eagan L. D., Thorpe T. A. Shikimate Pathway Activity during Shoot Initiation in Tobacco Callus Cultures. Plant Physiol. 1983 Oct;73(2):228–232. doi: 10.1104/pp.73.2.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bieleski R. L., Turner N. A. Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Anal Biochem. 1966 Nov;17(2):278–293. doi: 10.1016/0003-2697(66)90206-5. [DOI] [PubMed] [Google Scholar]
  3. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  4. FELS I. G. Acid-catalyzed oxidation of reduced pyridine nucleotides. Science. 1961 Jul 28;134(3474):280–281. doi: 10.1126/science.134.3474.280-a. [DOI] [PubMed] [Google Scholar]
  5. KOUKOL J., CONN E. E. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare. J Biol Chem. 1961 Oct;236:2692–2698. [PubMed] [Google Scholar]
  6. Kindl H. The regulation of the L-tyrosine ammonia-lyase activity by phenolic compounds. Hoppe Seylers Z Physiol Chem. 1970 Jul;351(7):792–798. doi: 10.1515/bchm2.1970.351.2.792. [DOI] [PubMed] [Google Scholar]
  7. Rao P. V., Moore K., Towers G. H. Degradation of aromatic amino acids by fungi. II. Purification and properties of phenylalanine ammonia-lyase from Ustilago hordei. Can J Biochem. 1967 Dec;45(12):1863–1872. doi: 10.1139/o67-219. [DOI] [PubMed] [Google Scholar]
  8. SKOOG F., MILLER C. O. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol. 1957;11:118–130. [PubMed] [Google Scholar]
  9. Thorpe T. A., Gaspar T. Changes in isoperoxidases during shoot formation in tobacco callus. In Vitro. 1978 Jun;14(6):522–526. doi: 10.1007/BF02616094. [DOI] [PubMed] [Google Scholar]

Articles from Plant Physiology are provided here courtesy of Oxford University Press

RESOURCES