Abstract
Selected variant cell lines of Haplopappus gracilis (Nutt) Gray that showed disturbed growth after transfer from an alanine medium to NO3− medium were characterized. The in vivo NO3− reductase activity (NRA) was lower in these lines than in the wild type. In vitro NRA assays suggest that decreased in vivo NRA was not caused by a lower amount of active enzyme. Cells of the variant lines revealed up to 75% lower extractable activity of NO2− reductase as compared with the wild type. This coincided with higher accumulation of NO2− by the variant than by the wild type cells after transfer from alanine medium to NO3− medium. NO2− accumulation was transient or continuous, depending on cell line, metabolic state of the cells, and light conditions.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Gamborg O. L., Miller R. A., Ojima K. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res. 1968 Apr;50(1):151–158. doi: 10.1016/0014-4827(68)90403-5. [DOI] [PubMed] [Google Scholar]
- Gupta S. C., Beevers L. Synthesis and degradation of nitrite reductase in pea leaves. Plant Physiol. 1984 May;75(1):251–252. doi: 10.1104/pp.75.1.251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jaworski E. G. Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun. 1971 Jun 18;43(6):1274–1279. doi: 10.1016/s0006-291x(71)80010-4. [DOI] [PubMed] [Google Scholar]
- Kelker H. C., Filner P. Regulation of nitrite reductase and its relationship to the regulation of nitrate reductase in cultured tobacco cells. Biochim Biophys Acta. 1971 Oct;252(1):69–82. doi: 10.1016/0304-4165(71)90093-6. [DOI] [PubMed] [Google Scholar]