Abstract
Many studies have shown that gibberellic acid (GA3) inhibits tuberization in potato (Solanum tuberosum L.). In this study, we have utilized the 40 kilodalton glycoprotein, patatin, as a marker for biochemical events associated with the process of tuberization. To determine the effects of exogenous applications of GA3 on the induction of the accumulation of this major tuber protein, we measured patatin levels in tubers from treated whole plants, petioles from a single-node cutting system with GA3 applied in a lanolin paste, and stolon tips cultured in vitro on an inductive medium supplemented with GA3. In all three systems, GA3 inhibited the accumulation of patatin and the major 15 and 22 kilodalton tuber proteins. This effect appeared to be selective since most of the other proteins were not affected and, in tubers, at least one protein was stimulated by GA3. These results suggest that GA3 can reverse biochemical events of tuberization in tubers as well as prevent the accumulation of the major tuber proteins in other inducible tissues.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
- Paiva E., Lister R. M., Park W. D. Induction and accumulation of major tuber proteins of potato in stems and petioles. Plant Physiol. 1983 Jan;71(1):161–168. doi: 10.1104/pp.71.1.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shurpalekar K. S., Singh N., Sundaravalli O. E. Nutritive value of leaf protein from lucerne (Medicago sativa). Growth responses in rats at different protein levels & to supplementation with lysine &-or methionine. Indian J Exp Biol. 1969 Oct;7(4):279–280. [PubMed] [Google Scholar]