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. 1985 Aug;78(4):849–852. doi: 10.1104/pp.78.4.849

Partial Purification and Characterization of Granule-Bound Starch Synthases from Normal and Waxy Maize 1

Fraser D Macdonald 1,2, Jack Preiss 1,3
PMCID: PMC1064836  PMID: 16664339

Abstract

The granule-bound starch-synthases from normal and waxy maize kernels have been solubilized, partially purified, and characterized. Two broad categories of starch synthases were revealed with representatives in the soluble phase and also on granules from both normal and waxy maize though the activity associated with granules from waxy was small. Data for native molecular weights, kinetic parameters, and immunological relatedness are used to demonstrate that the granule-bound isozymes from normal maize are different from the soluble enzymes. These distinct granule-bound enzymes are missing in waxy maize granules and a further novel form of starch synthase is revealed. These findings are discussed in relation to the type of starch produced in tissue affected by the waxy mutation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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