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. 1985 Aug;78(4):871–875. doi: 10.1104/pp.78.4.871

Membrane Transport in Isolated Vesicles from Sugarbeet Taproot 1

II. Evidence for a Sucrose/H+-Antiport

Donald P Briskin 1,2, W Robert Thornley 1,2, Roger E Wyse 1,2
PMCID: PMC1064840  PMID: 16664343

Abstract

The process of sucrose transport was investigated in sealed putative tonoplast vesicles isolated from sugarbeet (Beta vulgaris L.) taproot. If the vesicles were allowed to develop a steady state pH gradient by the associated transport ATPase and 10 millimolar sucrose was added, a transient flux of protons out of the vesicles was observed. The presence of an ATPase produced pH gradient allowed [14C]sucrose transport into the vesicles to occur at a rate 10-fold higher than the rate observed in the absence of an imposed pH gradient. Labeled sucrose accumulated into the sealed vesicles could be released back to the external medium if the pH gradient was dissipated with carbonylcyanide-m-chlorophenyl hydrazone (CCCP). When the kinetics of ATP dependent [14C]sucrose uptake were examined, the kinetic profile followed the simple Michaelis-Menten relationship and a Michaelis constant of 12.1 millimolar was found. When a transient, inwardly directed sucrose gradient was imposed on the vesicles in the absence of charge compensating ions, a transient interior negative membrane potential was observed. This membrane potential could be prevented by the addition of CCCP prior to sucrose or dissipated by the addition of CCCP after sucrose was added. These results suggest that an electrogenic H+/sucrose antiport may be operating on the vesicle membrane.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Briskin D. P., Thornley W. R., Wyse R. E. Membrane transport in isolated vesicles from sugarbeet taproot : I. Isolation and characterization of energy-dependent, h-transporting vesicles. Plant Physiol. 1985 Aug;78(4):865–870. doi: 10.1104/pp.78.4.865. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Giaquinta R. Sucrose Hydrolysis in Relation to Phloem Translocation in Beta vulgaris. Plant Physiol. 1977 Sep;60(3):339–343. doi: 10.1104/pp.60.3.339. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kaback H. R. The lac carrier protein in Escherichia coli. J Membr Biol. 1983;76(2):95–112. doi: 10.1007/BF02000610. [DOI] [PubMed] [Google Scholar]
  4. Kaczorowski G. J., Kaback H. R. Mechanism of lactose translocation in membrane vesicles from Escherichia coli. 1. Effect of pH on efflux, exchange, and counterflow. Biochemistry. 1979 Aug 21;18(17):3691–3697. doi: 10.1021/bi00584a009. [DOI] [PubMed] [Google Scholar]
  5. Kochian L. V., Lucas W. J. Potassium transport in corn roots : I. Resolution of kinetics into a saturable and linear component. Plant Physiol. 1982 Dec;70(6):1723–1731. doi: 10.1104/pp.70.6.1723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Maynard J. W., Lucas W. J. Sucrose and Glucose Uptake into Beta vulgaris Leaf Tissues : A Case for General (Apoplastic) Retrieval Systems. Plant Physiol. 1982 Nov;70(5):1436–1443. doi: 10.1104/pp.70.5.1436. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mitchell P. Vectorial chemistry and the molecular mechanics of chemiosmotic coupling: power transmission by proticity. Biochem Soc Trans. 1976;4(3):399–430. doi: 10.1042/bst0040399. [DOI] [PubMed] [Google Scholar]
  8. Roberts J. K., Wemmer D., Ray P. M., Jardetzky O. Regulation of Cytoplasmic and Vacuolar pH in Maize Root Tips under Different Experimental Conditions. Plant Physiol. 1982 Jun;69(6):1344–1347. doi: 10.1104/pp.69.6.1344. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Saftner R. A., Daie J., Wyse R. E. Sucrose uptake and compartmentation in sugar beet taproot tissue. Plant Physiol. 1983 May;72(1):1–6. doi: 10.1104/pp.72.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Saftner R. A., Wyse R. E. Alkali Cation/Sucrose Co-transport in the Root Sink of Sugar Beet. Plant Physiol. 1980 Nov;66(5):884–889. doi: 10.1104/pp.66.5.884. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Turner R. J. Quantitative studies of cotransport systems: models and vesicles. J Membr Biol. 1983;76(1):1–15. doi: 10.1007/BF01871450. [DOI] [PubMed] [Google Scholar]
  12. Waggoner A. S. Dye indicators of membrane potential. Annu Rev Biophys Bioeng. 1979;8:47–68. doi: 10.1146/annurev.bb.08.060179.000403. [DOI] [PubMed] [Google Scholar]
  13. Wyse R. Sucrose uptake by sugar beet tap root tissue. Plant Physiol. 1979 Nov;64(5):837–841. doi: 10.1104/pp.64.5.837. [DOI] [PMC free article] [PubMed] [Google Scholar]

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