Figure 2.

Clk^out mutant females have impaired exercise performance. At 5 days of age Clk^out females had significantly lower endurance than w¹¹¹⁸ controls, n = 8 vials of 20 flies each, (log-rank) (A). Clk^out females also had significantly reduced climbing speed, n = 5 vials of 20 flies each, (two-way ANOVA), line represents linear regression (B). Flight performance in Clk^out females was also significantly worse than controls, n = 8 vials of 20 flies each, (t-test) (C). Neither Clk^out females, nor w¹¹¹⁸ females showed any improvement in endurance with three weeks of endurance exercise training, n = 8 vials of 20 flies each, (log-rank analysis) (D). Control data displayed here, to allow for direct comparisons between untreated mutant and untreated control female flies, is also shown in rescue experiments in later figures. * p < 0.05, ** p < 0.001, ns is not significant.