Figure 6.

Octopamine feeding increases expression of exercise response genes in Clk^out mutants but does not impact Clock gene expression. qRT-PCR was used to evaluate expression of key genes in mutants and controls. Clk^out mutants had lower clock gene expression when compared to w¹¹¹⁸ controls, n = 3 biological replicates, (t-test) (A). OA feeding did not cause a significant change in the expression of clock in the mutants (B). However, OA feeding did lead to increased expression of both exercise response genes, sestrin and spargel, in both w¹¹¹⁸ (C) and Clk^out (D). The delta Ct method was used to compare gene expression between flies fed OA and those fed normal food, n = 3 biological replicates with three technical replicates within each biological replicate, t-test, * p < 0.05, ** p < 0.001.