Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (Danio rerio) | Tg(myl7: eGFP)twu34 | Huang et al., 2003 | twu34; RRID: ZFIN_ZDB-GENO-050809-10 |
Transgenic |
| Genetic reagent (Danio rerio) | Tg(sox17:eGFP)ha01 | Mizoguchi et al., 2008 | ha01; RRID: ZFIN_ZDB-GENO-080714-2 |
Transgenic |
| Genetic reagent (Danio rerio) | Tg(hsp70l:pdgfaa-2A-mCherry;cryaa:CFP)sd44 | Bloomekatz et al., 2017 | sd44; ZDB-ALT-170425-5 |
Transgenic |
| Genetic reagent (Danio rerio) | ref (pdgfrask16) | Bloomekatz et al., 2017 | sk16; ZDB-ALT-170329-1 |
Mutant |
| Genetic reagent (Danio rerio) | ptenahu1864 | Faucherre et al., 2008 | hu1864; ZDB-ALT-080910-1 |
Mutant |
| Genetic reagent (Danio rerio) | ptenbhu1435 | Faucherre et al., 2008 | hu1435; ZDB-ALT-080910-2 |
Mutant |
| Genetic reagent (Danio rerio) | Tg(myl7:dnPI3K; Cryaa:CFP) | This paper | Transgenic, see Materials and methods | |
| Genetic reagent (Danio rerio) | Tg(myl7:lck-emgfp) | This paper | Transgenic, see Materials and methods | |
| Recombinant DNA reagent | pBSRN3-∆p85 | Carballada et al., 2001 | ||
| Other | myl7 | Yelon et al., 1999 | ZDB-GENE-991019-3 | mRNA probe |
| Other | axial | Strähle et al., 1993 | ZDB-GENE-980526-404 | mRNA probe |
| Other | hand2 | Yelon et al., 2000 | ZDB-GENE-000511-1 | mRNA probe |
| Chemical compound, drug | LY294002 | Millipore-Sigma | Cat# 154447-36-6 | |
| Chemical compound, drug | Dactolisib | Millipore-Sigma | Cat# 915019-65-7 | |
| Chemical compound, drug | Pictilisib | Millipore-Sigma | Cat# 957054-30-7 | |
| Chemical compound, drug | Rapamycin | Selleckchem | Cat# S1039 | |
| Chemical compound, drug | VO-Ohpic trihydrate | Selleckchem | Cat# S8174 | |
| Antibody | phospho-AKT (Rabbit monoclonal) |
Cell Signaling | Cat# 4060, RRID: AB_2315049 | WB(1:2000) |
| Antibody | pan-AKT (Rabbit monoclonal) |
Cell Signaling | Cat# 4691, RRID: AB_915783 | WB (1:2000) |
| Antibody | Anti-rabbit IgG HRP-conjugated (Goat polyclonal) |
Cell Signaling | Cat# 7074, RRID: AB_2099233 | WB (1:5000) |
| Antibody | anti-GFP (Chicken polyclonal) |
Abcam | Cat# ab13970, RRID: AB_300798 |
IF (1:1000) |
| Antibody | anti-ZO-1 (Mouse monoclonal) |
Thermo Fisher Scientific | Cat# 33–9100, RRID: AB_87181 | IF (1:200) |
| Antibody | anti-chicken-488 (Goat polyclonal) |
Thermo Fisher Scientific | Cat# A32931TR, RRID: AB_2866499 | IF (1:300) |
| Antibody | anti-mouse-647 (Goat polyclonal) |
Thermo Fisher Scientific | Cat# A32728, RRID: AB_2633277 | IF (1:300) |
| Commercial assay or kit | Cell Death detection kit, TMR red | Millipore Sigma | Cat# 12156792910 | |
| Commercial assay or kit | Click-&-Go Cell Proliferation Assay Kit | Click Chemistry Tools | Cat# 1328 | |
| Software, algorithm | mTrackJ | Meijering et al., 2012 | ImageJ Plugin for motion tracking and analysis | |
| Software, algorithm | Correct 3D Drift | Parslow et al., 2014 | ImageJ Plugin for sample drift correction | |
| Software, algorithm | Prism 10.0.2 | GraphPad | RRID: SCR_002798 | Data visualization and statistics software |
| Software, algorithm | Leica | LASX | Leica Application Suite X, RRID: SCR_013673 | Microscope image processing software |