Fig 4.

BB0238 interacts with a periplasmic protein, BB0108. (A) The schema represents the experimental design of the BB0238 immunoprecipitation assay (IP). IP assays were performed on five separate groups of B. burgdorferi wild-type or bb0238 mutants (null or point mutants), as follows: Bb ^WT (with no antibody, control), Bb ^WT (with anti-BB0238), Bb ^BB0238− (with anti-BB0238), Bb ^Y37A (with anti-BB0238), and Bb ^P65A (with anti-BB0238). Eluted BB0238 interacting proteins were identified by liquid chromatography-MS/MS analysis. (B) Prediction of a model of BB0108 monomer by AlphaFold. The rainbow color scheme from blue to red corresponds to the tracing of the polypeptide chain from the N- to the C-terminus. Thus, the NC domain contains both red and blue colors, and the central PPIase domain is depicted in yellow and orange colors. (C) BB0108 monomer with colors corresponding to the confidence of the AlphaFold model from blue (highest confidence) to red (lowest confidence). The high confidence of the model is evidenced by having only blue colors. (D) Cartoon depiction of the AlphaFold model of the BB0108 dimer. Each monomer is shaded in a different color. The green molecule is shown in the same orientation as that of the monomer. (E) Surface representation of the dimer depicts the channel between the two protomers.