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. 1982 Sep;70(3):637–644. doi: 10.1104/pp.70.3.637

Functional Organization of the Chlorophyll-Containing Complexes of Chlamydomonas reinhardi1

A Study of Their Formation and Interconnection with Reaction Centers in the Greening Process of the y-1 Mutant

Jonathan M Gershoni 1,2, Susana Shochat 1,2, Shmuel Malkin 1,2, Itzhak Ohad 1,2
PMCID: PMC1065743  PMID: 16662548

Abstract

The stepwise synthesis and assembly of photosynthetic membrane components in the y-1 mutant of Chlamydomonas reinhardi have been previously demonstrated (Ohad 1975 In Membrane Biogenesis, Mitochondria, Chloroplasts and Bacteria, Plenum, pp 279-350). This experimental system was used here in order to investigate the process of formation and interconnection of the energy collecting chlorophylls with the reaction centers of both photosystems I and II. The following measurements were carried out: photosynthetic electron flow at various light intensities, including parts or the entire electron transfer chain; analysis of the kinetics of fluorescence emission at room temperature and fluorescence emission spectra at 77 K, and electrophoretic separation of membrane polypeptides and chlorophyll protein complexes. Based on the data obtained it is concluded that: (a) each photosystem (PSI and PSII) contains, in addition to the reaction center, an interconnecting antenna and a main or light harvesting antenna complex; (b) the formation of the light harvesting complex, interconnecting antenna, and reaction centers for each photosystem can occur independently. (c) the interconnecting antennae link the light harvesting complexes with the respective reaction centers. In their absence, energy transfer between the light harvesting chlorophylls and the reaction centers is inefficient. The formation of the interconnecting antennae and efficient assembly of photosystem components occur simultaneously with the de novo synthesis of chlorophyll and at least three polypeptides, one translated in the cytoplasm and two translated in the chloroplast. The synthesis of these polypeptides was found to be light dependent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acker S., Picaud A., Duranton J. Des activités photosynthétiques sans les complexes pigmentaires CP1 et CP2. Biochim Biophys Acta. 1976 Aug 13;440(2):269–277. doi: 10.1016/0005-2728(76)90061-x. [DOI] [PubMed] [Google Scholar]
  2. Bar-Nun S., Ohad I. Presence of Polypeptides of Cytoplasmic and Chloroplastic Origin in Isolated Photoactive Preparations of Photosystems I and II in Chlamydomonas reinhardi y-1. Plant Physiol. 1977 Feb;59(2):161–166. doi: 10.1104/pp.59.2.161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berzborn R. J., Bishop N. I. Isolation and properties of chloroplast particles of Scenedesmus obliquus D 3 with high photochemical activity. Biochim Biophys Acta. 1973 Apr 5;292(3):700–714. doi: 10.1016/0005-2728(73)90018-2. [DOI] [PubMed] [Google Scholar]
  4. Butler W. L., Strasser R. J. Tripartite model for the photochemical apparatus of green plant photosynthesis. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3382–3385. doi: 10.1073/pnas.74.8.3382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cahen D., Malkin S. Development of Photosystem II Complex during Greening of Chlamydomonas reinhardi y-1. Plant Physiol. 1976 Sep;58(3):257–267. doi: 10.1104/pp.58.3.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chua N. H., Bennoun P. Thylakoid membrane polypeptides of Chlamydomonas reinhardtii: wild-type and mutant strains deficient in photosystem II reaction center. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2175–2179. doi: 10.1073/pnas.72.6.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chua N. H., Gillham N. W. The sites of synthesis of the principal thylakoid membrane polypeptides in Chlamydomonas reinhardtii. J Cell Biol. 1977 Aug;74(2):441–452. doi: 10.1083/jcb.74.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chua N. H., Matlin K., Bennoun P. A chlorophyll-protein complex lacking in photosystem I mutants of Chlamydomonas reinhardtii. J Cell Biol. 1975 Nov;67(2PT1):361–377. doi: 10.1083/jcb.67.2.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Delepelaire P., Chua N. H. Lithium dodecyl sulfate/polyacrylamide gel electrophoresis of thylakoid membranes at 4 degrees C: Characterizations of two additional chlorophyll a-protein complexes. Proc Natl Acad Sci U S A. 1979 Jan;76(1):111–115. doi: 10.1073/pnas.76.1.111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Dubertret G., Ambard-Bretteville F. Functional and Structural Organization of Chlorophyll in the Developing Photosynthetic Membranes of Euglena gracilis Z: II. CHARACTERISTICS OF THE LATE FORMATION OF ACTIVE PHOTOSYSTEM II REACTION CENTERS DURING FIRST STAGES OF GREENING. Plant Physiol. 1981 Jan;67(1):47–53. doi: 10.1104/pp.67.1.47. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Dubertret G., Lefort-Tran M. Functional and structural organization of chlorophyll in the developing photosynthetic membranes of Euglena gracilis Z. IV. Light-harvesting properties of system II photosynthetic units and thylakoid ultrastructure during greening under intermittent light. Biochim Biophys Acta. 1981 Jan 14;634(1):52–69. doi: 10.1016/0005-2728(81)90127-4. [DOI] [PubMed] [Google Scholar]
  12. Gershoni J. M., Ohad I. The use of an internal standard for semiquantitative analysis of low temperature (77 degrees K) fluorescence of photosynthetic cells. Anal Biochem. 1980 May 15;104(2):315–320. doi: 10.1016/0003-2697(80)90081-0. [DOI] [PubMed] [Google Scholar]
  13. Gurevitz M., Kratz H., Ohad I. Polypeptides of chloroplastic and cytoplastic origin required for development of photosystem II activity, and chlorophyll-protein complexes, in Euglena gracilis Z chloroplast membranes. Biochim Biophys Acta. 1977 Sep 14;461(3):475–488. doi: 10.1016/0005-2728(77)90234-1. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Mullet J. E., Burke J. J., Arntzen C. J. Chlorophyll proteins of photosystem I. Plant Physiol. 1980 May;65(5):814–822. doi: 10.1104/pp.65.5.814. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Satoh K., Butler W. L. Low temperature spectral properties of subchloroplast fractions purified from spinach. Plant Physiol. 1978 Mar;61(3):373–379. doi: 10.1104/pp.61.3.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Thornber J. P., Alberte R. S., Hunter F. A., Shiozawa J. A., Kan K. S. The organization of chlorophyll in the plant photosynthetic unit. Brookhaven Symp Biol. 1976 Jun 7;(28):132–148. [PubMed] [Google Scholar]

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