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. 1982 Sep;70(3):649–654. doi: 10.1104/pp.70.3.649

Photosynthesis and Inorganic Carbon Transport in Isolated Asparagus Mesophyll Cells 1

George S Espie 1, Brian Colman 1
PMCID: PMC1065745  PMID: 16662550

Abstract

The possibility of HCO3− transport into isolated leaf mesophyll cells of Asparagus sprengeri Regel has been investigated. Measurement of the inorganic carbon pool in these cells over an external pH range 6.2 to 8.0, using the silicone-fluid filtration technique, indicated that the pool was larger than predicted by passive 14CO2 distribution, suggesting that HCO3− as well as CO2 crosses the plasmalemma. Intracellular pH values, calculated from the distribution of 14CO2 between the cells and the medium, were found to be higher (except at pH 8.0) than those previously determined by 5,5-dimethyl[2-14C]oxazolidine-2,4-dione distribution. It is suggested that the inorganic carbon accumulated above predicted concentrations may be bound to proteins and membranes and thus may not represent inorganic carbon actively accumulated by the cells, inasmuch as in a closed system at constant CO2 concentration, the photosynthetic rates at pH 7.0 and 8.0 were 5 to 8 times lower than the maximum rate which could be supported by CO2 arising from the spontaneous dehydration of HCO3−. Furthermore, CO2 compensation points of the cells in liquid media at 21% O2 at pH 7.0 and 8.0, and the K½ CO2 (CO2 concentration supporting the half maximal rate of O2 evolution) at 2% O2 at pH 7.0 and 8.0 are not consistent with HCO3− transport. These results indicate that the principal inorganic carbon species crossing the plasmalemma in these cells is CO2.

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Selected References

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