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. 1982 Sep;70(3):714–722. doi: 10.1104/pp.70.3.714

Immobilized Thylakoids in a Cross-Linked Albumin Matrix

Effects of Cations Studied by Electron Microscopy, Fluorescence Emission, Photoacoustic Spectroscopy, and Kinetic Measurements

Brigitte Thomasset 1,2,3, Thierry Thomasset 1,2,3, Alain Vejux 1,2,3, Joseph Jeanfils 1,2,3, Jean-Noël Barbotin 1,2,3, Daniel Thomas 1,2,3
PMCID: PMC1065758  PMID: 16662563

Abstract

Immobilization of lettuce (Lactuca sativa) thylakoids has been performed by using glutaraldehyde and bovine serum albumin. Confirming previous reports, a stabilization of the O2 evolution activity of the photosystem II (PSII) under storage and functional conditions has been observed. The present work is devoted to the role played by mono-and divalent cations, during the immobilization process itself, on the O2 production. Four types of measurements have been employed: kinetic measurements, low temperature (77 K) fluorescence emission, photoacoustic (PA) spectroscopy, and electron microscopy observations. We show that the effect of glutaraldehyde is complex because it acts as an inhibitor, a stabilizing agent, and a cross-linking reactive. In the present studies, the thylakoids are immobilized within a polymeric insoluble albumin matrix. The highest activity yield and the best storage conditions are obtained when 0.15 mm Na+ (or K+), 1 mm Mg2+, and 0.1 mm Mn2+ are present in the resuspending media before the immobilization. Due to modifications of the ionic content during such a process, structural differences are observed on the stacking degree of thylakoids. No modification of the fluorescence and PA spectra after the immobilization are found. Furthermore, a correlation between activities and spectral changes have been shown: when the activities increase, the F735 to F695 ratio increases and the PA676 to PA440 ratio decreases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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