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. 1982 Sep;70(3):749–753. doi: 10.1104/pp.70.3.749

Identification and Quantitative Analysis of Indole-3-Acetyl-l-Aspartate from Seeds of Glycine max L. 1

Jerry D Cohen 1,2
PMCID: PMC1065764  PMID: 16662569

Abstract

Indole-3-acetyl-l-aspartate (IAAsp) was isolated from seeds of Glycine max L. cv. Hark and its identity established by its chromatographic performance and its mass spectral fragmentation. Following acid hydrolysis, the aspartate moiety was shown to be the l-enantiomer by reverse phase high performance liquid chromatographic retention time of the bisethyl ester derivatized with 2,3,4,6-tetra-O-acetyl-β-d-glycopyranosyl isothiocyanate. Isotope dilution analysis using [14C]IAAsp as internal standard showed that soybean seed contained 10 μmol/kg IAAsp and this accounted for one-half of the total indoleacetic acid of the seed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andreae W. A., Good N. E. The Formation of Indoleacetylaspartic Acid in Pea Seedlings. Plant Physiol. 1955 Jul;30(4):380–382. doi: 10.1104/pp.30.4.380. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bandurski R. S., Schulze A. Concentration of Indole-3-acetic Acid and Its Derivatives in Plants. Plant Physiol. 1977 Aug;60(2):211–213. doi: 10.1104/pp.60.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cohen J. D., Schulze A. Double-standard isotope dilution assay. I. Quantitative assay of indole-3-acetic acid. Anal Biochem. 1981 Apr;112(2):249–257. doi: 10.1016/0003-2697(81)90290-6. [DOI] [PubMed] [Google Scholar]
  4. Davidonis G. H., Hamilton R. H., Mumma R. O. Metabolism of 2,4-dichlorophenoxyacetic Acid in soybean root callus and differentiated soybean root cultures as a function of concentration and tissue age. Plant Physiol. 1978 Jul;62(1):80–83. doi: 10.1104/pp.62.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ehmann A. The van urk-Salkowski reagent--a sensitive and specific chromogenic reagent for silica gel thin-layer chromatographic detection and identification of indole derivatives. J Chromatogr. 1977 Feb 11;132(2):267–276. doi: 10.1016/s0021-9673(00)89300-0. [DOI] [PubMed] [Google Scholar]
  6. Felker P. A gas--liquid chromatographic-isotope dilution analysis of cysteine, histidine, and tryptophan in acid-hydrolyzed protein. Anal Biochem. 1976 Nov;76(50):192–213. doi: 10.1016/0003-2697(76)90278-5. [DOI] [PubMed] [Google Scholar]
  7. Felker P., Bandurski R. S. Quantitative gas-liquid chromatography and mass spectrometry of the N(O)-perfluorobutyryl-O-isoamyl derivatives of amino acids. Anal Biochem. 1975 Jul;67(1):245–262. doi: 10.1016/0003-2697(75)90292-4. [DOI] [PubMed] [Google Scholar]
  8. Feung C. S., Hamilton R. H., Mumma R. O. Metabolism of Indole-3-acetic Acid: IV. Biological Properties of Amino Acid Conjugates. Plant Physiol. 1977 Jan;59(1):91–93. doi: 10.1104/pp.59.1.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Feung C. S., Hamilton R. H., Witham F. H., Mumma R. O. The relative amounts and identification of some 2,4-dichlorophenoxyacetic Acid metabolites isolated from soybean cotyledon callus cultures. Plant Physiol. 1972 Jul;50(1):80–86. doi: 10.1104/pp.50.1.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Feung C., Hamilton R. H., Mumma R. O. Metabolism of 2,4-dichlorophenoxyacetic acid. V. Identification of metabolites in soybean callus tissue cultures. J Agric Food Chem. 1973 Jul-Aug;21(4):637–640. doi: 10.1021/jf60188a058. [DOI] [PubMed] [Google Scholar]
  11. Good N. E., Andreae W. A., Ysselstein M. W. Studies on 3-Indoleacetic Acid Metabolism. II. Some Products of the Metabolism of Exogenous Indoleacetic Acid in Plant Tissues. Plant Physiol. 1956 May;31(3):231–235. doi: 10.1104/pp.31.3.231. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hangarter R. P., Good N. E. Evidence That IAA Conjugates Are Slow-Release Sources of Free IAA in Plant Tissues. Plant Physiol. 1981 Dec;68(6):1424–1427. doi: 10.1104/pp.68.6.1424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Olney H. O. Growth substances from Veratrum tenuipetalum. Plant Physiol. 1968 Mar;43(3):293–302. doi: 10.1104/pp.43.3.293. [DOI] [PMC free article] [PubMed] [Google Scholar]

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