Abstract
When seeds of soybeans (Glycine max Amsoy var.) or safflower were stored under high O2 concentrations, their per cent germination declined rapidly. For example, soybean seeds stored under 7.7 atmospheres O2 pressure at 25°C and 17% moisture lost all viability within 22 days, whereas under 7.7 atmospheres N2, the per cent germination remained greater than 80%. Germination decreased continually in O2 pressures ranging from 0 to 7.7 atmospheres. High levels of O2, moisture, or temperature each acted independently to cause losses of germination, but when applied simultaneously, these factors acted synergistically. Soybean seeds were also aged under conditions of high temperature (44°C) and humidity (100% RH), which have been routinely used to accelerate aging. Under these conditions, no O2 dependence of seed death was observable.
Increased lipid oxidation was not detected in seeds that had lost germination ability due to high O2 treatment. Seeds of two safflower varieties that contained either high oleic or high linoleic fatty acid compositions were subjected to high O2 treatment. Although the lipid of the high oleic variety is markedly more stable to oxidative degradation, we detected no significant difference in the O2 tolerance of these seeds.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dillard C. J., Tappel A. L. Fluorescent products from reaction of peroxidizing polyunsaturated fatty acids with phosphatidyl ethanolamine and phenylalanine. Lipids. 1973 Apr;8(4):183–189. doi: 10.1007/BF02544632. [DOI] [PubMed] [Google Scholar]
- Hara A., Radin N. S. Lipid extraction of tissues with a low-toxicity solvent. Anal Biochem. 1978 Oct 1;90(1):420–426. doi: 10.1016/0003-2697(78)90046-5. [DOI] [PubMed] [Google Scholar]
- Pammenter N. W., Adamson J. H., Berjak P. Viability of stored seed: extension by cathodic protection. Science. 1974 Dec 20;186(4169):1123–1124. doi: 10.1126/science.186.4169.1123. [DOI] [PubMed] [Google Scholar]
- Parrish D. J., Leopold A. C. On the mechanism of aging in soybean seeds. Plant Physiol. 1978 Mar;61(3):365–368. doi: 10.1104/pp.61.3.365. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Priestley D. A., Leopold A. C. Absence of Lipid Oxidation during Accelerated Aging of Soybean Seeds. Plant Physiol. 1979 Apr;63(4):726–729. doi: 10.1104/pp.63.4.726. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart R. R., Bewley J. D. Lipid peroxidation associated with accelerated aging of soybean axes. Plant Physiol. 1980 Feb;65(2):245–248. doi: 10.1104/pp.65.2.245. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Trombly R., Tappel A. Fractionation and analysis of fluorescent products of lipid peroxidation. Lipids. 1975 Aug;10(8):441–447. doi: 10.1007/BF02532426. [DOI] [PubMed] [Google Scholar]