Abstract
Phytochrome was localized by immunoelectron microscopy in cells of the coleoptile tip of etiolated and irradiated oat (Avena sativa L., cv. Konata) seedlings. By using ultrathin frozen sections and immunopurified, monospecific antibodies, both the sensitivity and resolution of the immunocytochemical assay were increased. The results with etiolated plants agree with and extend previously published data. A brief red light illumination caused the redistribution of phytochrome from a diffuse to a more particulate appearance. Areas that accumulated phytochrome were identified as small vacuoles into which phytochrome was sequestered following illumination. In seedlings illuminated for several hours and in normal light-grown plants, the cellular distribution of phytochrome is qualitatively similar to that of nonirradiated, dark-grown material, except that in green plants the nucleus shows a positive immunocytochemical reaction.
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- Coleman R. A., Pratt L. H. Electron microscopic localization of phytochrome in plants using an indirect antibody-labeling method. J Histochem Cytochem. 1974 Nov;22(11):1039–1047. doi: 10.1177/22.11.1039. [DOI] [PubMed] [Google Scholar]
- Hunt R. E., Pratt L. H. Phytochrome immunoaffinity purification. Plant Physiol. 1979 Aug;64(2):332–336. doi: 10.1104/pp.64.2.332. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mackenzie J. M., Jr, Coleman R. A., Briggs W. R., Pratt L. H. Reversible redistribution of phytochrome within the cell upon conversion to its physiologically active form. Proc Natl Acad Sci U S A. 1975 Mar;72(3):799–803. doi: 10.1073/pnas.72.3.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pratt L. H., Coleman R. A. Immunocytochemical localization of phytochrome. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2431–2435. doi: 10.1073/pnas.68.10.2431. [DOI] [PMC free article] [PubMed] [Google Scholar]