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. 1982 Oct;70(4):927–931. doi: 10.1104/pp.70.4.927

Photosynthetic Oxygen Exchange in Isolated Cells and Chloroplasts of C3 Plants

Robert T Furbank 1, Murray R Badger 1, C Barry Osmond 1
PMCID: PMC1065801  PMID: 16662645

Abstract

Photosynthetic O2-production and photorespiratory O2-uptake were measured, using stable isotope techniques, in isolated intact leaf cells of the C3 plant Xanthium strumarium L., and isolated intact chloroplasts of Spinacia oleracea L (var. Yates 102). Considerable light dependent O2-uptake was observed in both systems, a proportion of which could be suppressed by CO2 (63% suppression in chloroplasts by 50 micromolar CO2, 58% in cells by 100 micromolar CO2 and 250 micromolar O2). At low O2, O2-uptake was CO2 insensitive. At high CO2 up to 19% of total electron flow was to O2 in cells and up to 14% in chloroplasts. O2-uptake showed inhibition by KCN (61% in cells, 35% in chloroplasts by 0.2 millimolar KCN). O2-uptake half saturated at 75 to 85 micromolar O2 in cells and 50 to 65 micromolar O2 in chloroplasts, at low CO2. The results are discussed in terms of the RuP2-oxygenase reaction and direct photoreduction of O2 via a Mehler reaction.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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