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. 1982 Oct;70(4):978–981. doi: 10.1104/pp.70.4.978

Chloroplast Sulfhydryl Groups and the Light Activation of Fructose-1,6-Bisphosphatase 1

Rudolf E Slovacek 1, Sharon Vaughn 1
PMCID: PMC1065810  PMID: 16662654

Abstract

Studies of isolated intact spinach (Spinacia oleracea L.) chloroplasts reveal that most of the available sulfhydryl groups are associated with stromal protein as opposed to a thylakoid membrane fraction under non-denaturing conditions. Increases in sulfhydryl content of approximately 50% occurred with illumination and could be correlated kinetically with a reductive activation of fructose-1,6-bisphosphatase during CO2-assimilation. Inhibition of linear electron flow with 3-(3,4-dichlorophenyl)-1,1-dimethylurea prevented light driven increases in both fructose-1,6-bisphosphatase activity and the relative sulfhydryl number. These results provide evidence for the operation of a reductive enzyme activating system in vivo.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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